BV-2 microglial cells sense micro-nanotextured silicon surface topology

Saydulla Persheyev*, Yongchang Fan, Andrew Irving, Mervyn J. Rose

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Artificial biomimetic substrates provide useful models for studying cell adhesion, signaling, and differentiation. This article describes biological interactions with a new type of tunable, micro-nanotextured silicon substrate, generated by irradiation of a hydrogenated amorphous silicon film with a large beam, excimer laser (248 nm). In this study, we demonstrate that BV-2 microglial cells can sense differences in laser processed silicon surface topology over the range of 30 nm to 2 μm, where they undergo marked morphogenic changes with increasing feature size. The cells adopt a more elongated shape in the presence of the modified surface structure and exhibit increased levels of actin-rich microdomains, suggesting enhanced adhesion. The excimer laser modification of hydrogenated amorphous silicon to generate micro-nanostructures realizes large area benefits as well as providing a biomaterial where the external and internal structure can be altered and tuned for various applications.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume99 A
Issue number1
DOIs
Publication statusPublished - Oct 2011

Keywords

  • actin
  • BV-2 microglia cells
  • cell adhesion
  • cell signaling and interactions
  • cytoskeleton
  • micro-nanotextured silicon

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