Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase

Xiaohong Shi; Catherine Helen Botting; Ping Li; Mark Niglas; Benjamin Brennan; Sally Lorna Shirran; Agnieszka Marta Szemiel; Richard Michael Elliott

doi:10.1073/pnas.1603364113

Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase

Xiaohong Shi, Catherine Helen Botting, Ping Li, Mark Niglas, Benjamin Brennan, Sally Lorna Shirran, Agnieszka Marta Szemiel, Richard Michael Elliott

Research output: Contribution to journal › Article › peer-review

Abstract

Bunyamwera virus (BUNV) is the prototype of the Orthobunyavirus genus and Bunyaviridae family that contains important human and animal pathogens. The cleavage mechanism of orthobunyavirus glycoprotein precursor (GPC) and the host proteases involved have not been clarified. Here we found that NSm and Gc contain their own internal signal peptides, which mediate the GPC cleavage by host signal peptidase and signal peptide peptidase (SPP). Furthermore, the NSm domain-I plays an important postcleavage role in cell fusion. Our data clarified the implication of host proteases in the processing of the orthobunyavirus GPC. This work identifies SPP as a potential intervention target, and the knowledge we gained will benefit preventive strategies against other orthobunyavirus infections.

Original language	English
Pages (from-to)	8825-8830
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	113
Issue number	31
Early online date	20 Jul 2016
DOIs	https://doi.org/10.1073/pnas.1603364113
Publication status	Published - 2 Aug 2016

Keywords

Bunyavirua
Bunyamwera virus
Glycoprotein precursor processing
Signal peptidase
Signal peptide peptidase

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10.1073/pnas.1603364113

Shi_2016_PNAS_Cleavage_AAM
© 2016, the Author(s). This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at www.pnas.org / https://dx.doi.org/10.1073/pnas.1603364113
Accepted author manuscript, 845 KB

MaXis ESI QTOF mass spectrometer: Equipment only grant-Mass spectrometers for Proteomics, Lipidomics and focused Metabolomics research
Botting, C. H. (PI), Elliott, R. M. (CoI), Randall, R. E. (CoI), Smith, T. K. (CoI) & White, M. (CoI)
The Wellcome Trust
1/08/11 → 31/07/14
Project: Standard

Cite this

Shi, X., Botting, C. H., Li, P., Niglas, M., Brennan, B., Shirran, S. L., Szemiel, A. M., & Elliott, R. M. (2016). Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase. Proceedings of the National Academy of Sciences of the United States of America, 113(31), 8825-8830. https://doi.org/10.1073/pnas.1603364113

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title = "Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase",

abstract = "Bunyamwera virus (BUNV) is the prototype of the Orthobunyavirus genus and Bunyaviridae family that contains important human and animal pathogens. The cleavage mechanism of orthobunyavirus glycoprotein precursor (GPC) and the host proteases involved have not been clarified. Here we found that NSm and Gc contain their own internal signal peptides, which mediate the GPC cleavage by host signal peptidase and signal peptide peptidase (SPP). Furthermore, the NSm domain-I plays an important postcleavage role in cell fusion. Our data clarified the implication of host proteases in the processing of the orthobunyavirus GPC. This work identifies SPP as a potential intervention target, and the knowledge we gained will benefit preventive strategies against other orthobunyavirus infections. ",

keywords = "Bunyavirua, Bunyamwera virus, Glycoprotein precursor processing, Signal peptidase, Signal peptide peptidase",

author = "Xiaohong Shi and Botting, {Catherine Helen} and Ping Li and Mark Niglas and Benjamin Brennan and Shirran, {Sally Lorna} and Szemiel, {Agnieszka Marta} and Elliott, {Richard Michael}",

note = "This study was supported by Wellcome Trust Grant 099220/B/12/Z (to R.M.E.) and Grant 094476/Z/10/Z that funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex (BSRC) of University of St. Andrews.",

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Shi, X, Botting, CH, Li, P, Niglas, M, Brennan, B, Shirran, SL, Szemiel, AM & Elliott, RM 2016, 'Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 31, pp. 8825-8830. https://doi.org/10.1073/pnas.1603364113

Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase. / Shi, Xiaohong; Botting, Catherine Helen; Li, Ping et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 31, 02.08.2016, p. 8825-8830.

Research output: Contribution to journal › Article › peer-review

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T1 - Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase

AU - Shi, Xiaohong

AU - Botting, Catherine Helen

AU - Li, Ping

AU - Niglas, Mark

AU - Brennan, Benjamin

AU - Shirran, Sally Lorna

AU - Szemiel, Agnieszka Marta

AU - Elliott, Richard Michael

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AB - Bunyamwera virus (BUNV) is the prototype of the Orthobunyavirus genus and Bunyaviridae family that contains important human and animal pathogens. The cleavage mechanism of orthobunyavirus glycoprotein precursor (GPC) and the host proteases involved have not been clarified. Here we found that NSm and Gc contain their own internal signal peptides, which mediate the GPC cleavage by host signal peptidase and signal peptide peptidase (SPP). Furthermore, the NSm domain-I plays an important postcleavage role in cell fusion. Our data clarified the implication of host proteases in the processing of the orthobunyavirus GPC. This work identifies SPP as a potential intervention target, and the knowledge we gained will benefit preventive strategies against other orthobunyavirus infections.

KW - Bunyavirua

KW - Bunyamwera virus

KW - Glycoprotein precursor processing

KW - Signal peptidase

KW - Signal peptide peptidase

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DO - 10.1073/pnas.1603364113

M3 - Article

SN - 1091-6490

VL - 113

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 31

ER -

Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase

Abstract

Keywords

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MaXis ESI QTOF mass spectrometer: Equipment only grant-Mass spectrometers for Proteomics, Lipidomics and focused Metabolomics research

Cite this