Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase

Xiaohong Shi, Catherine Helen Botting, Ping Li, Mark Niglas, Benjamin Brennan, Sally Lorna Shirran, Agnieszka Marta Szemiel, Richard Michael Elliott

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)
2 Downloads (Pure)

Abstract

Bunyamwera virus (BUNV) is the prototype of the Orthobunyavirus genus and Bunyaviridae family that contains important human and animal pathogens. The cleavage mechanism of orthobunyavirus glycoprotein precursor (GPC) and the host proteases involved have not been clarified. Here we found that NSm and Gc contain their own internal signal peptides, which mediate the GPC cleavage by host signal peptidase and signal peptide peptidase (SPP). Furthermore, the NSm domain-I plays an important postcleavage role in cell fusion. Our data clarified the implication of host proteases in the processing of the orthobunyavirus GPC. This work identifies SPP as a potential intervention target, and the knowledge we gained will benefit preventive strategies against other orthobunyavirus infections.
Original languageEnglish
Pages (from-to)8825-8830
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number31
Early online date20 Jul 2016
DOIs
Publication statusPublished - 2 Aug 2016

Keywords

  • Bunyavirua
  • Bunyamwera virus
  • Glycoprotein precursor processing
  • Signal peptidase
  • Signal peptide peptidase

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