Binding of a cellular factor to the 3 ' untranslated region of the RNA genomes of entero- and rhinoviruses plays a role in virus replication

KH Mellits, JM Meredith, JB Rohll, DJ Evans, JW Almond*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The presence of cellular factors that bind to the 3' untranslated region (UTR) of picornaviruses was investigated by electrophoretic mobility shift assays (EMSAs). A cellular factor(s) that binds specifically the 3' UTR of polio-, coxsackie-and rhinoviruses was detected. Furthermore, this factor(s) is distinct from those which bind to the 5' terminal 88 nt (the 'cloverleaf') of poliovirus. Mutations within the 3' UTR which decrease the affinity of the RNA for the cellular factor in EMSAs decrease RNA replication and virus viability. Revertants of these mutants display changes which are predicted to stabilize the RNA secondary structure of the 3' UTR. These results indicate that binding of a cellular factor to the UTR plays a role in virus replication and that RNA secondary structure is important for this function.

Original languageEnglish
Pages (from-to)1715-1723
Number of pages9
JournalJournal of General Virology
Volume79
Publication statusPublished - Jul 1998

Keywords

  • POLIOVIRUS RNA
  • NUCLEOTIDE-SEQUENCE
  • TRANSLATION
  • INITIATION
  • COMPLEXES
  • FEATURES
  • END

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