BacA is essential for bacteroid development in nodules of galegoid, but not phaseoloid, legumes

Ramakrishnan Karunakaran, Andreas F Haag, Alison K East, Vinoy K Ramachandran, Jurgen Prell, Euan K James, Marco Scocchi, Gail P Ferguson, Philip S Poole

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

BacA is an integral membrane protein, the mutation of which leads to increased resistance to the antimicrobial peptides bleomycin and Bac7(1-35) and a greater sensitivity to SDS and vancomycin in Rhizobium leguminosarum bv. viciae, R. leguminosarum bv. phaseoli, and Rhizobium etli. The growth of Rhizobium strains on dicarboxylates as a sole carbon source was impaired in bacA mutants but was overcome by elevating the calcium level. While bacA mutants elicited indeterminate nodule formation on peas, which belong to the galegoid tribe of legumes, bacteria lysed after release from infection threads and mature bacteroids were not formed. Microarray analysis revealed almost no change in a bacA mutant of R. leguminosarum bv. viciae in free-living culture. In contrast, 45 genes were more-than 3-fold upregulated in a bacA mutant isolated from pea nodules. Almost half of these genes code for cell membrane components, suggesting that BacA is crucial to alterations that occur in the cell envelope during bacteroid development. In stark contrast, bacA mutants of R. leguminosarum bv. phaseoli and R. etli elicited the formation of normal determinate nodules on their bean host, which belongs to the phaseoloid tribe of legumes. Bacteroids from these nodules were indistinguishable from the wild type in morphology and nitrogen fixation. Thus, while bacA mutants of bacteria that infect galegoid or phaseoloid legumes have similar phenotypes in free-living culture, BacA is essential only for bacteroid development in indeterminate galegoid nodules.

Original languageEnglish
Pages (from-to)2920-8
Number of pages9
JournalJournal of Bacteriology
Volume192
Issue number11
DOIs
Publication statusPublished - 1 Jun 2010

Keywords

  • Bacterial Proteins/genetics
  • Fabaceae/microbiology
  • Gene Expression Regulation, Bacterial/genetics
  • Microbial Sensitivity Tests
  • Oligonucleotide Array Sequence Analysis
  • Peas/microbiology
  • Rhizobium leguminosarum/genetics

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