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Abstract
The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.
[GRAPHICS]
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A neurite that has had its shape detected and trajectory to an arbitrary target point computed. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Original language | English |
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Pages (from-to) | 682-692 |
Number of pages | 11 |
Journal | Journal of Biophotonics |
Volume | 2 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2009 |
Keywords
- neuron growth
- spatial light modulator
- Bessel beam
- HOLOGRAPHIC OPTICAL TWEEZERS
- COMPUTER-GENERATED HOLOGRAMS
- FEMTOSECOND LASER
- CELLS
- MICROSCOPY
- TRAP
- MICROMANIPULATION
- TRANSFECTION
- MANIPULATION
- PARTICLES
Fingerprint
Dive into the research topics of 'Automated laser guidance of neuronal growth cones using a spatial light modulator'. Together they form a unique fingerprint.Projects
- 2 Finished
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EP/C536037/1: Advanced Biophotonics Workstation
Dholakia, K. (PI), Gunn-Moore, F. J. (CoI), Riches, A. C. (CoI) & Sibbett, W. (CoI)
1/10/05 → 30/09/09
Project: Standard
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EP/C536037/1: Advanced Biophotonics Workstation
Riches, A. C. (PI), Dholakia, K. (CoI), Gunn-Moore, F. J. (CoI) & Sibbett, W. (CoI)
1/10/05 → 30/09/09
Project: Standard