Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

Edwin Garcia; Annette Hayden; Charles Birts; Edward Britton; Andrew Cowie; Karen Pickard; Massimiliano Mellone; Clarisa Choh; Mathieu Derouet; Patrick Duriez; Fergus Noble; Michael J. White; John N. Primrose; Jonathan C. Strefford; Matthew Rose-Zerilli; Gareth J. Thomas; Yeng Ang; Andrew D. Sharrocks; Rebecca C. Fitzgerald; Timothy J. Underwood; OCCAMS Consortium

doi:10.1038/srep32417

Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

Edwin Garcia, Annette Hayden, Charles Birts, Edward Britton, Andrew Cowie, Karen Pickard, Massimiliano Mellone, Clarisa Choh, Mathieu Derouet, Patrick Duriez, Fergus Noble, Michael J. White, John N. Primrose, Jonathan C. Strefford, Matthew Rose-Zerilli, Gareth J. Thomas, Yeng Ang, Andrew D. Sharrocks, Rebecca C. Fitzgerald, Timothy J. Underwood^*OCCAMS Consortium

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.

Original language	English
Article number	32417
Number of pages	12
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep32417
Publication status	Published - 7 Sept 2016

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Garcia, E., Hayden, A., Birts, C., Britton, E., Cowie, A., Pickard, K., Mellone, M., Choh, C., Derouet, M., Duriez, P., Noble, F., White, M. J., Primrose, J. N., Strefford, J. C., Rose-Zerilli, M., Thomas, G. J., Ang, Y., Sharrocks, A. D., Fitzgerald, R. C., ... OCCAMS Consortium (2016). Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1. Scientific Reports, 6, Article 32417. https://doi.org/10.1038/srep32417

@article{4dece57159c640b89b5465f70b64b806,

title = "Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1",

abstract = "New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.",

author = "Edwin Garcia and Annette Hayden and Charles Birts and Edward Britton and Andrew Cowie and Karen Pickard and Massimiliano Mellone and Clarisa Choh and Mathieu Derouet and Patrick Duriez and Fergus Noble and White, {Michael J.} and Primrose, {John N.} and Strefford, {Jonathan C.} and Matthew Rose-Zerilli and Thomas, {Gareth J.} and Yeng Ang and Sharrocks, {Andrew D.} and Fitzgerald, {Rebecca C.} and Underwood, {Timothy J.} and {OCCAMS Consortium} and Lynch, {Andy G.}",

year = "2016",

month = sep,

day = "7",

doi = "10.1038/srep32417",

language = "English",

volume = "6",

journal = "Scientific Reports",

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Garcia, E, Hayden, A, Birts, C, Britton, E, Cowie, A, Pickard, K, Mellone, M, Choh, C, Derouet, M, Duriez, P, Noble, F, White, MJ, Primrose, JN, Strefford, JC, Rose-Zerilli, M, Thomas, GJ, Ang, Y, Sharrocks, AD, Fitzgerald, RC, Underwood, TJ & OCCAMS Consortium 2016, 'Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1', Scientific Reports, vol. 6, 32417. https://doi.org/10.1038/srep32417

TY - JOUR

T1 - Authentication and characterisation of a new oesophageal adenocarcinoma cell line

T2 - MFD-1

AU - Garcia, Edwin

AU - Hayden, Annette

AU - Birts, Charles

AU - Britton, Edward

AU - Cowie, Andrew

AU - Pickard, Karen

AU - Mellone, Massimiliano

AU - Choh, Clarisa

AU - Derouet, Mathieu

AU - Duriez, Patrick

AU - Noble, Fergus

AU - White, Michael J.

AU - Primrose, John N.

AU - Strefford, Jonathan C.

AU - Rose-Zerilli, Matthew

AU - Thomas, Gareth J.

AU - Ang, Yeng

AU - Sharrocks, Andrew D.

AU - Fitzgerald, Rebecca C.

AU - Underwood, Timothy J.

AU - OCCAMS Consortium

AU - Lynch, Andy G.

PY - 2016/9/7

Y1 - 2016/9/7

N2 - New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.

AB - New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.

U2 - 10.1038/srep32417

DO - 10.1038/srep32417

M3 - Article

AN - SCOPUS:84987680020

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 32417

ER -

Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

Abstract

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