Assessment of murine collagen-induced arthritis by longitudinal non-invasive duplexed molecular optical imaging

Hannah E. Scales, Michelle Ierna, Karen M. Smith, Kirsty Ross, Gordon R. Meiklejohn, Janet C. Patterson-Kane, Iain B. McInnes, James M. Brewer, Paul Garside, Pasquale Maffia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Objective. In the present study we evaluated the use of four commercially available fluorescent probes to monitor disease activity in murine CIA and its suppression during glucocorticoid therapy.

Methods. Arthritis was induced in male DBA/1 mice by immunization with type II collagen in Complete Freund’s Adjuvant, followed by a boost of collagen in PBS. Four fluorescent probes from PerkinElmer in combination [ProSense 750 fluorescent activatable sensor technology (FAST) with Neutrophil Elastase 680 FAST and MMPSense 750 FAST with CatK 680 FAST] were used to monitor disease development from day 5 through to day 40 post-immunization. Fluorescence generated in vivo by the probes was correlated with clinical and histological score and paw measurements.

Results. The fluorescence intensity emitted by each probe was shown to correlate with the conventional measurements of disease. The highest degree of correlation was observed with ProSense 750 FAST in combination with Neutrophil Elastase 680 FAST; these probes were then used to successfully assess CIA suppression during dexamethasone treatment.

Conclusion. We have demonstrated that longitudinal non-invasive duplexed optical fluorescence imaging provides a simple assessment of arthritic disease activity within the joints of mice following the induction of CIA and may represent a powerful t
Original languageEnglish
Pages (from-to)564-572
Number of pages9
JournalRheumatology
Volume55
Issue number3
Early online date16 Oct 2015
DOIs
Publication statusPublished - 16 Oct 2015

Keywords

  • CatK 680 FAST
  • Collagen-induced arthritis
  • Dexamethasone
  • MMPSense 750 FAST
  • Neutrophil elastase 680 FAST
  • Optical imaging
  • ProSense 750 FAST

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