Artificial metalloenzymes as catalysts for oxidative lignin degradation

Megan Doble, Amanda Jarvis, Andy Ward, Jonathan David Colburn, Jan Philipp Goetze, Michael Buehl, Paul C J Kamer

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
3 Downloads (Pure)

Abstract

We report novel artificial metalloenzymes (ArMs), containing tris(pyridylmethyl)amine (TPA), for the atom economic oxidation of lignin β-O-4 model compounds, using hydrogen peroxide. The protein scaffold alters the selectivity of the reaction from a low yielding cleavage reaction when using the parent Fe-tpa complex to a high yielding benzylic alcohol oxidation when using the complex incorporated into a protein scaffold, SCP-2L A100C. Engineering the protein scaffold to incorporate glutamic acid was found to improve the ArM activity, showing that rational design of the protein environment using metal binding amino acids can be a first step towards improving the overall activity of an artificial metalloenzyme.
Original languageEnglish
Pages (from-to)15100-15107
Number of pages8
JournalACS Sustainable Chemistry & Engineering
Volume6
Issue number11
Early online date10 Oct 2018
DOIs
Publication statusPublished - 5 Nov 2018

Keywords

  • Lignin
  • Artificial metalloenzymes
  • Catalytic oxidation

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