Analytical method to determine the orientation of rigid spin labels in DNA

Andriy Marko, Dominik Margraf, Pavol Cekan, Snorri Th. Sigurdsson, Olav Schiemann, Thomas F. Prisner

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

We demonstrate the ability of pulsed electron double resonance (PELDOR) experiments to determine the orientation of spin labels in biological macromolecules. Thus, the distance information usually obtained from PELDOR data can be complemented by the mutual orientation of macromolecular domains. A method to determine the angle beta between the spin label normal and the interspin axis is proposed and analyzed mathematically. The obtained analytical expression allows extraction of angles beta without a fitting procedure if these angles are equal for both nitroxide of biradical. The method was applied to the experimental data gathered on ten spin-labeled DNA samples. The angles estimated from the PELDOR data are in excellent agreement with literature values.

Original languageEnglish
Article number021911
Number of pages9
JournalPhysical Review. E, Statistical, nonlinear, and soft matter physics
Volume81
Issue number2
DOIs
Publication statusPublished - Feb 2010

Keywords

  • ELECTRON-PARAMAGNETIC-RESONANCE
  • PAIR DISTANCE DISTRIBUTIONS
  • EPR SPECTROSCOPY
  • NUCLEIC-ACIDS
  • RIBONUCLEOTIDE REDUCTASE
  • TIKHONOV REGULARIZATION
  • DISORDERED SOLIDS
  • RADICAL PAIRS
  • PULSED ESR
  • PELDOR

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