Abstract
We demonstrate the ability of pulsed electron double resonance (PELDOR) experiments to determine the orientation of spin labels in biological macromolecules. Thus, the distance information usually obtained from PELDOR data can be complemented by the mutual orientation of macromolecular domains. A method to determine the angle beta between the spin label normal and the interspin axis is proposed and analyzed mathematically. The obtained analytical expression allows extraction of angles beta without a fitting procedure if these angles are equal for both nitroxide of biradical. The method was applied to the experimental data gathered on ten spin-labeled DNA samples. The angles estimated from the PELDOR data are in excellent agreement with literature values.
Original language | English |
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Article number | 021911 |
Number of pages | 9 |
Journal | Physical Review. E, Statistical, nonlinear, and soft matter physics |
Volume | 81 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 2010 |
Keywords
- ELECTRON-PARAMAGNETIC-RESONANCE
- PAIR DISTANCE DISTRIBUTIONS
- EPR SPECTROSCOPY
- NUCLEIC-ACIDS
- RIBONUCLEOTIDE REDUCTASE
- TIKHONOV REGULARIZATION
- DISORDERED SOLIDS
- RADICAL PAIRS
- PULSED ESR
- PELDOR