An optical trapping apparatus incorporating violet diode laser and extended cavity surface emitting laser systems

TK Lake; AE Carruthers; L Paterson; M Taylor; Francis James Gunn-Moore; JW Allen; Wilson Sibbett; Kishan Dholakia

An optical trapping apparatus incorporating violet diode laser and extended cavity surface emitting laser systems

TK Lake, AE Carruthers, L Paterson, M Taylor, Francis James Gunn-Moore, JW Allen, Wilson Sibbett, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics. (C) 2004 Optical Society of America.

Original language	English
Pages (from-to)	670-8
Number of pages	9
Journal	Optics Express
Volume	12
Issue number	4
Publication status	Published - 23 Feb 2004

Keywords

DIELECTRIC PARTICLES
TWEEZERS
FORCES
CELLS

Cite this

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keywords = "DIELECTRIC PARTICLES, TWEEZERS, FORCES, CELLS",

author = "TK Lake and AE Carruthers and L Paterson and M Taylor and Gunn-Moore, {Francis James} and JW Allen and Wilson Sibbett and Kishan Dholakia",

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AU - Lake, TK

AU - Carruthers, AE

AU - Paterson, L

AU - Taylor, M

AU - Gunn-Moore, Francis James

AU - Allen, JW

AU - Sibbett, Wilson

AU - Dholakia, Kishan

N1 - Highlighted in Biophotonics international Impact factor - 2.3

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AB - Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics. (C) 2004 Optical Society of America.

KW - DIELECTRIC PARTICLES

KW - TWEEZERS

KW - FORCES

KW - CELLS

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M3 - Article

SN - 1094-4087

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JO - Optics Express

JF - Optics Express

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An optical trapping apparatus incorporating violet diode laser and extended cavity surface emitting laser systems

Abstract

Keywords

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