An improved assay for radiation-induced chromatid breaks using a colcemid block and calyculin-induced PCC combination

O Shovman, Andrew Clive Riches, D Adamson, Peter Edward Bryant

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is used alone for a longer period and produces metaphase spreads with superior quality. By this procedure, the chromosomes and the chromatid breaks are expanded and thereby make for improved break scoring. In a comparison of the new technique with the conventional colcemid block method, we show a close proportionality between the frequencies of chromatid breaks scored with the two methods. The frequency of chromatid breaks with the new method was found to be significantly higher than that with colcemid alone, adding a higher sensitivity to the assay as an additional advantage.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalMutagenesis
Volume23
Issue number4
Early online date6 Mar 2008
DOIs
Publication statusPublished - Jul 2008

Keywords

  • G2 CHROMOSOMAL RADIOSENSITIVITY
  • CANCER-PATIENTS
  • GENETIC PREDISPOSITION
  • REPAIR
  • CELLS
  • LYMPHOCYTES
  • CONDENSATION
  • MARKER
  • VARIABILITY
  • SENSITIVITY

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