An extremely thermostable aldolase from Sulfolobus solfataricus with specificity for non-phosphorylated substrates

C.L. Buchanan, H. Connaris, M.J. Danson, C.D. Reeve, D.W. Hough

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

Sulfolobus solfataricus is a hyperthermophilic archaeon growing optimally at 80-85°C. It metabolizes glucose via a novel non-phosphorylated Entner-Doudoroff pathway, in which the reversible C to C aldol cleavage is catalysed by 2-keto-3-deoxygluconate aldolase (KDG-aldolase), generating pyruvate and glyceraldehyde. Given the ability of such a hyperstable enzyme to catalyse carbon-carbon-bond synthesis with non-phosphorylated metabolites, we report here the cloning and sequencing of the S. solfataricus gene encoding KDG-aldolase, and its expression in Escherichia coli to give fully active enzyme. The recombinant enzyme was purified in a simple two-step procedure, and shown to possess kinetic properties indistinguishable from the enzyme purified from S. solfataricus cells. The KDG-aldolase is a thermostable tetrameric protein with a halflife at 100°C of 2.5 h, and is equally active with both D- and L-glyceraldehyde. It exhibits sequence similarity to the N-acetylneuraminate lyase superfamily of Schiff-base-dependent aldolases, dehydratases and decarboxylases, and evidence is presented for a similar catalytic mechanism for the archaeal enzyme by substrate-dependent inactivation by reduction with NaBH.

Original languageEnglish
Pages (from-to)563-570
Number of pages8
JournalBiochemical Journal
Volume343
Issue number3
DOIs
Publication statusPublished - 1 Nov 1999

Keywords

  • Biotransformation
  • C-C bond
  • 2-keto-3-deoxy-gluconate

Fingerprint

Dive into the research topics of 'An extremely thermostable aldolase from Sulfolobus solfataricus with specificity for non-phosphorylated substrates'. Together they form a unique fingerprint.

Cite this