An efficient method to express GPI-anchor proteins in insect cells

Hosam Shams-Eldin, Nahid Azzouz, Sebastian Niehus, Terry K. Smith, Ralph T. Schwarz

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Glycosylphosphatidylinositols (GPIs) constitute a class of glycolipids that have various functions, the most basic being to attach proteins to the surface of eukaryotic cells. GPIs have to be taken into account, when expressing surface antigens from parasitic protozoa in heterologous systems. The synthesis of the GPI-anchors was previously reported to be drastically decreased to almost background level following baculovirus infection. Here we describe a new method to express GPI-anchor proteins in insect cells relying on using of a supplementary baculovirus construct that overexpresses the N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the enzyme catalyzing the second step in the GPI biosynthetic pathway. (c) 2007 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)657-663
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume365
DOIs
Publication statusPublished - 25 Jan 2008

Keywords

  • baculovirus
  • insect cells
  • Sf9
  • high five
  • glycosylphosphatidylinositols
  • N-glycosylation
  • toxoplasma gondii
  • N-acetylglucosaminyl phosphatidylinositol de-n-acetylase
  • PIGL
  • SAG1
  • MEROZOITE SURFACE PROTEIN-1
  • GLYCOSYLPHOSPHATIDYLINOSITOL BIOSYNTHETIC-PATHWAY
  • DE-N-ACETYLASE
  • PLASMODIUM-FALCIPARUM
  • RECOMBINANT GLYCOPROTEINS
  • INHIBITORY ANTIBODIES
  • BACULOVIRUS INFECTION
  • TRYPANOSOMA-BRUCEI
  • TOXOPLASMA-GONDII
  • MAMMALIAN-CELLS

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