All-optical assay to study biological neural networks

Wardiya Afshar Saber; Federico M. Gasparoli; Marjet Gabrielle Dirks; Frank J. Gunn-Moore; Maciej Antkowiak

doi:10.3389/fnins.2018.00451

All-optical assay to study biological neural networks

Wardiya Afshar Saber, Federico M. Gasparoli, Marjet Gabrielle Dirks, Frank J. Gunn-Moore, Maciej Antkowiak

Research output: Contribution to journal › Article › peer-review

Abstract

We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.

Original language	English
Article number	451
Number of pages	12
Journal	Frontiers in Neuroscience
Volume	12
DOIs	https://doi.org/10.3389/fnins.2018.00451
Publication status	Published - 5 Jul 2018

Keywords

All-optical assay
Optogenetics
Calcium imaging
Neural networks
Connectivity
Neurons
Lentiviral transduction

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10.3389/fnins.2018.00451Licence: CC BY

AfsharSaber_2018_FN_All-opticalassay_CC
© 2018 Afshar Saber, Gasparoli, Dirks, Gunn-Moore and Antkowiak. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Final published version, 2.46 MBLicence: CC BY

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Gunn-Moore, F. J. (PI)
EPSRC
1/08/17 → 31/07/22
Project: Standard
Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Dholakia, K. (PI) & Gather, M. C. (CoI)
1/08/17 → 31/07/22
Project: Standard

Cite this

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title = "All-optical assay to study biological neural networks",

abstract = "We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity. ",

keywords = "All-optical assay, Optogenetics, Calcium imaging, Neural networks, Connectivity, Neurons, Lentiviral transduction",

author = "{Afshar Saber}, Wardiya and Gasparoli, {Federico M.} and Dirks, {Marjet Gabrielle} and Gunn-Moore, {Frank J.} and Maciej Antkowiak",

note = "This work was supported by the Cunningham Trust Ph.D. Studentship, the RS MacDonald Charitable Trust Neurophotonics Grant and EPSRC programme Grant EP/P030017/1.",

year = "2018",

month = jul,

day = "5",

doi = "10.3389/fnins.2018.00451",

language = "English",

volume = "12",

journal = "Frontiers in Neuroscience",

issn = "1662-453X",

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T1 - All-optical assay to study biological neural networks

AU - Afshar Saber, Wardiya

AU - Gasparoli, Federico M.

AU - Dirks, Marjet Gabrielle

AU - Gunn-Moore, Frank J.

AU - Antkowiak, Maciej

N1 - This work was supported by the Cunningham Trust Ph.D. Studentship, the RS MacDonald Charitable Trust Neurophotonics Grant and EPSRC programme Grant EP/P030017/1.

PY - 2018/7/5

Y1 - 2018/7/5

N2 - We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.

AB - We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.

KW - All-optical assay

KW - Optogenetics

KW - Calcium imaging

KW - Neural networks

KW - Connectivity

KW - Neurons

KW - Lentiviral transduction

U2 - 10.3389/fnins.2018.00451

DO - 10.3389/fnins.2018.00451

M3 - Article

SN - 1662-453X

VL - 12

JO - Frontiers in Neuroscience

JF - Frontiers in Neuroscience

M1 - 451

ER -

All-optical assay to study biological neural networks

Abstract

Keywords

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Projects

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world

Cite this