Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

James P. C.  Coverdale; Hugo A. van den Berg; Siavash Khazaipoul; Hannah E. Bridgewater; Alan J. Stewart; Claudia A. Blindauer

doi:10.1039/D2CC02278H

Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

James P. C. Coverdale, Hugo A. van den Berg, Siavash Khazaipoul, Hannah E. Bridgewater, Alan J. Stewart, Claudia A. Blindauer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.

Original language	English
Pages (from-to)	7384-7387
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	53
Early online date	13 Jun 2022
DOIs	https://doi.org/10.1039/D2CC02278H
Publication status	Published - 7 Jul 2022

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10.1039/D2CC02278HLicence: CC BY

Coverdale_2022_CC_Albumin_CC
Copyright © The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Final published version, 2.04 MBLicence: CC BY

Bilateral BBSRC-FAPESP: Bilateral BBSRC-FAPESP: A "speciomic" toolkit to investigate fatty acid-mediated changes in plasma zinc speciation and their physiological effects
Stewart, A. J. (PI)
BBSRC
1/11/21 → 31/10/24
Project: Standard
A Quantitive framework linking: A Quantitive framework linking extracellular zinc speciation and cell uptake
Stewart, A. J. (PI)
The Leverhulme Trust
4/09/17 → 3/09/20
Project: Standard
Dynamics of Circulatory Zinc Handling: The Dynamics of Circulatory Zinc Handling and Transport
Stewart, A. J. (PI)
BBSRC
9/04/12 → 8/04/15
Project: Standard

Cite this

@article{072f016dc1cb44b88fe91af514b607d6,

title = "Albumin-mediated extracellular zinc speciation drives cellular zinc uptake",

abstract = "The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.",

author = "Coverdale, {James P. C.} and {van den Berg}, {Hugo A.} and Siavash Khazaipoul and Bridgewater, {Hannah E.} and Stewart, {Alan J.} and Blindauer, {Claudia A.}",

note = "This work was financially supported by the Leverhulme Trust (RPG-2017-214) and BBSRC (BB/J006467/1 and BB/V014684/1). We thank Prof. Andrew Riches (University of St. Andrews) for provision of materials, and Dr. Elizabeth Bolitho (University of Warwick) for assistance with cell culture experiments.",

year = "2022",

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doi = "10.1039/D2CC02278H",

language = "English",

volume = "58",

pages = "7384--7387",

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publisher = "Royal Society of Chemistry",

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T1 - Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

AU - Coverdale, James P. C.

AU - van den Berg, Hugo A.

AU - Khazaipoul, Siavash

AU - Bridgewater, Hannah E.

AU - Stewart, Alan J.

AU - Blindauer, Claudia A.

N1 - This work was financially supported by the Leverhulme Trust (RPG-2017-214) and BBSRC (BB/J006467/1 and BB/V014684/1). We thank Prof. Andrew Riches (University of St. Andrews) for provision of materials, and Dr. Elizabeth Bolitho (University of Warwick) for assistance with cell culture experiments.

PY - 2022/7/7

Y1 - 2022/7/7

N2 - The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.

AB - The role of the extracellular medium in influencing metal uptake into cells has not been described quantitatively. In a chemically defined model system containing albumin, zinc influx into endothelial cells correlates with the extracellular free zinc concentration. Allosteric inhibition of zinc-binding to albumin by free fatty acids increased zinc flux.

U2 - 10.1039/D2CC02278H

DO - 10.1039/D2CC02278H

M3 - Article

SN - 1359-7345

VL - 58

SP - 7384

EP - 7387

JO - Chemical Communications

JF - Chemical Communications

IS - 53

ER -

Albumin-mediated extracellular zinc speciation drives cellular zinc uptake

Abstract

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Projects

Bilateral BBSRC-FAPESP: Bilateral BBSRC-FAPESP: A "speciomic" toolkit to investigate fatty acid-mediated changes in plasma zinc speciation and their physiological effects

A Quantitive framework linking: A Quantitive framework linking extracellular zinc speciation and cell uptake

Dynamics of Circulatory Zinc Handling: The Dynamics of Circulatory Zinc Handling and Transport

Cite this