Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

Marius Haugland; Janet Eleanor Lovett; Edward Anderson

doi:10.1039/C6CS00550K

Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

Marius Haugland, Janet Eleanor Lovett, Edward Anderson

Research output: Contribution to journal › Review article › peer-review

92 Citations (Scopus)

5 Downloads (Pure)

Abstract

EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.

Original language	English
Pages (from-to)	668-680
Journal	Chemical Society Reviews
Volume	47
Issue number	3
Early online date	1 Dec 2017
DOIs	https://doi.org/10.1039/C6CS00550K
Publication status	Published - 7 Feb 2018

Keywords

Nitroxide spin labels
EPR spectroscopy
Chemical synthesis

Access to Document

10.1039/C6CS00550K

ChemSocRev_accepted
© 2017, the Author(s). This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1039/C6CS00550K
Accepted author manuscript, 1.52 MB

New Strategies for Spin Labelling: New strategies for spin labelling cysteine-rich proteins
Lovett, J. E. (PI)
EPSRC
1/09/14 → 31/08/15
Project: Standard
Royal Society Research Fellowship: Royal Society Research Fellowship
Lovett, J. E. (PI)
The Royal Society
1/05/14 → 15/02/17
Project: Fellowship

Cite this

@article{aa80cea5291e44d2890fdc2d9d246578,

title = "Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling",

abstract = "EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.",

keywords = "Nitroxide spin labels, EPR spectroscopy, Chemical synthesis",

author = "Marius Haugland and Lovett, {Janet Eleanor} and Edward Anderson",

note = "Funding: UK EPSRC (EP/L022044/1), and the Royal Society for a University Research Fellowship (JEL).",

year = "2018",

month = feb,

day = "7",

doi = "10.1039/C6CS00550K",

language = "English",

volume = "47",

pages = "668--680",

journal = "Chemical Society Reviews",

issn = "0306-0012",

publisher = "Royal Society of Chemistry",

number = "3",

}

TY - JOUR

T1 - Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

AU - Haugland, Marius

AU - Lovett, Janet Eleanor

AU - Anderson, Edward

N1 - Funding: UK EPSRC (EP/L022044/1), and the Royal Society for a University Research Fellowship (JEL).

PY - 2018/2/7

Y1 - 2018/2/7

N2 - EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.

AB - EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.

KW - Nitroxide spin labels

KW - EPR spectroscopy

KW - Chemical synthesis

U2 - 10.1039/C6CS00550K

DO - 10.1039/C6CS00550K

M3 - Review article

SN - 0306-0012

VL - 47

SP - 668

EP - 680

JO - Chemical Society Reviews

JF - Chemical Society Reviews

IS - 3

ER -

Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

Abstract

Keywords

Access to Document

Fingerprint

Projects

New Strategies for Spin Labelling: New strategies for spin labelling cysteine-rich proteins

Royal Society Research Fellowship: Royal Society Research Fellowship

Cite this