Advances in the synthesis of nitroxide radicals for use in biomolecule spin labelling

Marius Haugland, Janet Eleanor Lovett, Edward Anderson

Research output: Contribution to journalReview articlepeer-review

57 Citations (Scopus)

Abstract

EPR spectroscopy is an increasingly useful analytical tool to probe biomolecule structure, dynamic behaviour, and interactions. Nitroxide radicals are the most commonly used radical probe in EPR experiments, and many methods have been developed for their synthesis, as well as incorporation into biomolecules using site-directed spin labelling. In this Tutorial Review, we discuss the most practical methods for the synthesis of nitroxides, focusing on the tunability of their structures, the manipulation of their sidechains into spin labelling handles, and their installation into biomolecules.
Original languageEnglish
Pages (from-to)668-680
JournalChemical Society Reviews
Volume47
Issue number3
Early online date1 Dec 2017
DOIs
Publication statusPublished - 7 Feb 2018

Keywords

  • Nitroxide spin labels
  • EPR spectroscopy
  • Chemical synthesis

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