Advanced fluorescence microscopy methods for the real-time study of transcription and chromatin dynamics

Paolo Annibale, Enrico Gratton*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

6 Citations (Scopus)

Abstract

In this contribution we provide an overview of the recent advances allowed by the use of fluorescence microscopy methods in the study of transcriptional processes and their interplay with the chromatin architecture in living cells. Although the use of fluorophores to label nucleic acids dates back at least to about half a century ago,1 two recent breakthroughs have effectively opened the way to use fluorescence routinely for specific and quantitative probing of chromatin organization and transcriptional activity in living cells: namely, the possibility of labeling (i)the chromatin loci and (ii) the mRNA synthesized from a gene using fluorescent proteins. In this contribution we focus on methods that can probe rapid dynamic processes by analyzing fast fluorescence fluctuations.

Original languageEnglish
Article numbere28425
JournalTranscription
Volume5
Issue numberMAR
DOIs
Publication statusPublished - 2014

Keywords

  • 3D orbital tracking
  • Brightness an
  • Fluctuations
  • Fluorescence
  • Pair correlation function
  • RICS

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