Advanced EPR spectroscopy for investigation of biomolecular binding events

Joshua Wort, Maria Oranges, Katrin Ackermann, Bela Ernest Bode

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The emergence of systems biology in the post-genomic era has led to investigating increasingly complex macromolecular assemblies, emphasising holistic approaches in structural biology. This changing philosophy is prefaced on the understanding that pathologies are often borne-out of dysregulated, multifaceted interactions within wider biological networks. However, while accurate characterisation of such biomolecular interactions in a biologically valid context is important, this dramatically increases the experimental complexity. In this purview, electron paramagnetic resonance (EPR) is particularly appealing because it is one of few techniques not overwhelmed by the increasing complexity of biomacromolecules in their native context or supramolecular networks. In a variety of ways EPR can additionally detect and quantify interactions, intimately coupling structural information and binding events within the biological or structural context.
Original languageEnglish
Title of host publicationElectron Paramagnetic Resonance
Subtitle of host publicationVolume 27
EditorsDamien Murphy, Victor Chechik, Bela Bode
PublisherRoyal Society of Chemistry (RSC)
Chapter2
Pages47-73
Number of pages27
Volume27
ISBN (Electronic)9781839163173
ISBN (Print)9781839161711
DOIs
Publication statusPublished - 2020

Publication series

NameSPR - Electron Paramagnetic Resonance

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