Abstract
Replication of the adenovirus genome is catalysed by adenovirus DNA polymerase in which the adenovirus preterminal protein acts as a protein primer. DNA polymerase and preterminal protein form a heterodimer which, in the presence of the cellular transcription factors NFI/CTFI and NFIII/Oct-1, binds to the origin of DNA replication. DNA replication is initiated by DNA polymerase mediated transfer of dCMP onto preterminal protein. Further DNA synthesis is catalysed by DNA polymerase in a strand displacement mechanism which also requires adenovirus DNA binding protein. Here, we discuss the role of individual proteins in this process as revealed by biochemical analysis, mutagenesis and molecular modelling.
| Original language | English |
|---|---|
| Volume | 272 |
| Publication status | Published - 2003 |
Keywords
- NUCLEAR FACTOR-I
- TERMINAL PROTEIN-PRECURSOR
- SINGLE-STRANDED-DNA
- LINKER-INSERTION MUTAGENESIS
- TRANSCRIPTION FACTORS NFI
- ANIMAL VIRUS-DNA
- BINDING-PROTEIN
- PRETERMINAL PROTEIN
- VIRAL-DNA
- CRYSTAL-STRUCTURE