Activation of hatching in diapaused and quiescent Globodera pallida

Juan E. Palomares-Rius*, John T. Jones, Peter J. Cock, Pablo Castillo, Vivian C. Blok

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis are major pests of potatoes. The G. pallida (and G. rostochiensis) life cycle includes both diapause and quiescent stages. Nematodes in dormancy (diapause or quiescent) are adapted for long-term survival and are more resistant to nematicides. This study analysed the mechanisms underlying diapause and quiescence. The effects of several compounds (8Br-cGMP, oxotremorine and atropine) on the activation of hatching were studied. The measurements of some morphometric parameters in diapaused and quiescent eggs after exposure to PRD revealed differences in dorsal gland length, subventral gland length and dorsal gland nucleolus. In addition, the expression of 2 effectors (IVg9 and cellulase) was not induced in diapaused eggs in water or PRD, while expression was slightly induced in quiescent eggs. Finally, we performed a comparative study to identify orthologues of C. elegans diapause related genes in plant-parasitic nematodes (G. pallida, Meloidogyne incognita, M. hapla and Bursaphelenchus xylophilus). This analysis suggested that it was not possible to identify G. pallida orthologues of the majority of C. elegans genes involved in the control of dauer formation. All these data suggest that G. pallida may use different mechanisms to C. elegans in regulating the survival stage.

Original languageEnglish
Pages (from-to)445-454
Number of pages10
JournalParasitology
Volume140
Issue number4
DOIs
Publication statusPublished - Apr 2013

Keywords

  • hatching
  • cGMP
  • muscarinic pathway
  • potato cyst nematodes
  • root diffusate
  • ANCYLOSTOMA-CANINUM
  • INFECTIVE LARVAE
  • CAENORHABDITIS-ELEGANS
  • ROSTOCHIENSIS
  • IDENTIFICATION
  • NEMATODES
  • EVOLUTION
  • SEQUENCE
  • PATHWAY
  • STAGE

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