Accurate extraction of nanometer distances in multimers by pulse EPR

Silvia Valera; Katrin Ackermann; Christos Pliotas; Hexian Huang; James Henderson Naismith; Bela Ernest Bode

doi:10.1002/chem.201505143

Accurate extraction of nanometer distances in multimers by pulse EPR

Silvia Valera
, Katrin Ackermann
, Christos Pliotas
, Hexian Huang
, James Henderson Naismith
, Bela Ernest Bode

Research output: Contribution to journal › Article › peer-review

Abstract

Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in
structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane embedded ion channels where data analysis is commonly hindered by multi-spin effects.

Original language	English
Pages (from-to)	4700–4703
Journal	Chemistry - A European Journal
Volume	22
Issue number	14
Early online date	25 Feb 2016
DOIs	https://doi.org/10.1002/chem.201505143
Publication status	Published - 16 Mar 2016

Keywords

Biomimetic synthesis
Deer
EPR spectroscopy
Ion channels
Mambrane proteins
Structural biology

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10.1002/chem.201505143Licence: CC BY

Valera_et_al-2016-ChemistryEurJ_CC-BY
Copyright 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.37 MBLicence: CC BY

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Intra-monomer EPR: Intra-monomer EPR distances in multimeric systems
Bode, B. (PI)
EPSRC
14/08/15 → 13/08/16
Project: Standard
Spin-labelled terpyridines: Spin-labelled terpyridines as model complexes for biomolecular dimerisation studied via nanometre distance measurements by pulsed electron paramagnetic resonance
Bode, B. (PI)
The Royal Society
16/03/15 → 15/03/16
Project: Standard
Oligo PELDOR: Oligo PELDOR
Bode, B. (PI)
Joint Research Centre European Commission
1/03/13 → 28/02/17
Project: Fellowship

Bela Bode
- beb2st-andrews.acuk
Person: Academic

Data Underpinning - Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR
Valera, S. (Creator), Ackermann, K. (Creator), Pliotas, C. (Creator), Huang, H. (Creator), Naismith, J. H. (Creator) & Bode, B. E. (Creator), University of St Andrews, 19 Feb 2016
DOI: 10.17630/C6FEB988-D15C-4B53-9CD9-02E9610C75EC
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Cite this

@article{695395b26a62454abd073f590a3c04e8,

title = "Accurate extraction of nanometer distances in multimers by pulse EPR",

abstract = "Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane embedded ion channels where data analysis is commonly hindered by multi-spin effects.",

keywords = "Biomimetic synthesis, Deer, EPR spectroscopy, Ion channels, Mambrane proteins, Structural biology",

author = "Silvia Valera and Katrin Ackermann and Christos Pliotas and Hexian Huang and Naismith, \{James Henderson\} and Bode, \{Bela Ernest\}",

note = "B.E.B. is grateful for funding from the European Union (REA 334496). This work was supported by the EPSRC (EP/M024660/1), the Wellcome Trust [099149/Z/12/Z], and the Royal Society (RG140723).",

year = "2016",

month = mar,

day = "16",

doi = "10.1002/chem.201505143",

language = "English",

volume = "22",

pages = "4700–4703",

journal = "Chemistry - A European Journal",

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publisher = "John Wiley \& Sons, Ltd",

number = "14",

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TY - JOUR

T1 - Accurate extraction of nanometer distances in multimers by pulse EPR

AU - Valera, Silvia

AU - Ackermann, Katrin

AU - Pliotas, Christos

AU - Huang, Hexian

AU - Naismith, James Henderson

AU - Bode, Bela Ernest

N1 - B.E.B. is grateful for funding from the European Union (REA 334496). This work was supported by the EPSRC (EP/M024660/1), the Wellcome Trust [099149/Z/12/Z], and the Royal Society (RG140723).

PY - 2016/3/16

Y1 - 2016/3/16

N2 - Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane embedded ion channels where data analysis is commonly hindered by multi-spin effects.

AB - Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane embedded ion channels where data analysis is commonly hindered by multi-spin effects.

KW - Biomimetic synthesis

KW - Deer

KW - EPR spectroscopy

KW - Ion channels

KW - Mambrane proteins

KW - Structural biology

U2 - 10.1002/chem.201505143

DO - 10.1002/chem.201505143

M3 - Article

SN - 0947-6539

VL - 22

SP - 4700

EP - 4703

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

IS - 14

ER -

Accurate extraction of nanometer distances in multimers by pulse EPR

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Intra-monomer EPR: Intra-monomer EPR distances in multimeric systems

Spin-labelled terpyridines: Spin-labelled terpyridines as model complexes for biomolecular dimerisation studied via nanometre distance measurements by pulsed electron paramagnetic resonance

Oligo PELDOR: Oligo PELDOR

Profiles

Bela Bode

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Data Underpinning - Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR

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