A type III-B CRISPR–Cas effector complex mediating massive target DNA destruction

Wenyuan Han, Yingjun Li, Ling Deng, Mingxia Feng, Wenfang Peng, Søren Hallstrøm, Jing Zhang, Nan Peng, Yun Xiang Liang, Malcolm Frederick White, Qunxin She*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)
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Abstract

The CRISPR (clustered regularly interspaced short palindromic repeats) system protects archaea and bacteria by eliminating nucleic acid invaders in a crRNA-guided manner. The Sulfolobus islandicus type III-B effector Cmr-α complex targets invading nucleic acid at both RNA and DNA levels and DNA targeting relies on the directional transcription of the protospacer in vivo. To gain further insight into the involved mechanism, we purified a native effector complex of III-B Cmr-α from S. islandicus and characterized it in vitro. Cmr-α cleaved RNAs complementary to crRNA present in the complex and its ssDNA destruction activity was activated by target RNA. ssDNA cleavage required mismatches between the 5’-tag of crRNA and the 3’-flanking region of target RNA. An invader plasmid assay showed that mutation either in the HD domain (a quadruple mutation) or in the GGDD motif of theCmr-2α protein resulted in attenuation of the DNA interference in vivo. However, double mutation of the HD motif only abolished the DNase activity in vitro. Furthermore, the activated Cmr-α binary complex functioned as a highly active DNase to destroy a large excess of substrate, which could provide a powerful means to rapidly degrade replicating viral DNA.
Original languageEnglish
Pages (from-to)1983-1993
Number of pages11
JournalNucleic Acids Research
Volume45
Issue number4
Early online date15 Dec 2016
DOIs
Publication statusPublished - 28 Feb 2017

Keywords

  • CRISPR-Cas
  • RNA-activated DNA cleavage
  • Dual nucleic acids interference
  • Cas10
  • Sulfolobus islandicus

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