A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

H.J. Lamble; S.F Royer; D.W. Hough; M.J. Danson; Garry Lindsay Taylor; S.D. Bull

doi:10.1002/adsc.200600520

A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

H.J. Lamble, S.F Royer, D.W. Hough, M.J. Danson, Garry Lindsay Taylor, S.D. Bull

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.

Original language	English
Pages (from-to)	817-821
Number of pages	5
Journal	Advanced Synthesis & Catalysis
Volume	349
DOIs	https://doi.org/10.1002/adsc.200600520
Publication status	Published - Apr 2007

Keywords

aldol reaction
aldolase
3-deoxy-2-ulosonic acids
diastereoselectivity
pyruvate
SULFOLOBUS-SOLFATARICUS
DIRECTED EVOLUTION
2-KETO-3-DEOXYGLUCONATE ALDOLASE
PSEUDOMONAS SACCHAROPHILA
EFFICIENT SYNTHESIS
KDO ALDOLASE
DERIVATIVES
ENZYMES
DAH
TRANSFORMATION

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10.1002/adsc.200600520

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title = "A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids",

abstract = "A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.",

keywords = "aldol reaction, aldolase, 3-deoxy-2-ulosonic acids, diastereoselectivity, pyruvate, SULFOLOBUS-SOLFATARICUS, DIRECTED EVOLUTION, 2-KETO-3-DEOXYGLUCONATE ALDOLASE, PSEUDOMONAS SACCHAROPHILA, EFFICIENT SYNTHESIS, KDO ALDOLASE, DERIVATIVES, ENZYMES, DAH, TRANSFORMATION",

author = "H.J. Lamble and S.F Royer and D.W. Hough and M.J. Danson and Taylor, {Garry Lindsay} and S.D. Bull",

year = "2007",

month = apr,

doi = "10.1002/adsc.200600520",

language = "English",

volume = "349",

pages = "817--821",

journal = "Advanced Synthesis & Catalysis",

issn = "1615-4150",

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T1 - A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

AU - Lamble, H.J.

AU - Royer, S.F

AU - Hough, D.W.

AU - Danson, M.J.

AU - Taylor, Garry Lindsay

AU - Bull, S.D.

PY - 2007/4

Y1 - 2007/4

N2 - A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.

AB - A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.

KW - aldol reaction

KW - aldolase

KW - 3-deoxy-2-ulosonic acids

KW - diastereoselectivity

KW - pyruvate

KW - SULFOLOBUS-SOLFATARICUS

KW - DIRECTED EVOLUTION

KW - 2-KETO-3-DEOXYGLUCONATE ALDOLASE

KW - PSEUDOMONAS SACCHAROPHILA

KW - EFFICIENT SYNTHESIS

KW - KDO ALDOLASE

KW - DERIVATIVES

KW - ENZYMES

KW - DAH

KW - TRANSFORMATION

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U2 - 10.1002/adsc.200600520

DO - 10.1002/adsc.200600520

M3 - Article

SN - 1615-4150

VL - 349

SP - 817

EP - 821

JO - Advanced Synthesis & Catalysis

JF - Advanced Synthesis & Catalysis

ER -

A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

Abstract

Keywords

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