A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

David Jackson; A  Cadman; T  Zurcher; W S  Barclay

doi:10.1128/JVI.76.22.11744-11747.2002

A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

David Jackson, A Cadman, T Zurcher, W S Barclay

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

Original language	English
Pages (from-to)	11744-11747
Number of pages	4
Journal	Journal of Virology
Volume	76
Issue number	22
DOIs	https://doi.org/10.1128/JVI.76.22.11744-11747.2002
Publication status	Published - Nov 2002

Keywords

A VIRUS
MODEL RNA
NONCODING REGIONS
IN-VIVO
EXPRESSION
NEURAMINIDASE
REPLICATION
RESISTANCE
POLYMERASE
MUTATIONS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.76.22.11744-11747.2002

Cite this

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title = "A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA",

abstract = "The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.",

keywords = "A VIRUS, MODEL RNA, NONCODING REGIONS, IN-VIVO, EXPRESSION, NEURAMINIDASE, REPLICATION, RESISTANCE, POLYMERASE, MUTATIONS",

author = "David Jackson and A Cadman and T Zurcher and Barclay, {W S}",

year = "2002",

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doi = "10.1128/JVI.76.22.11744-11747.2002",

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T1 - A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

AU - Jackson, David

AU - Cadman, A

AU - Zurcher, T

AU - Barclay, W S

PY - 2002/11

Y1 - 2002/11

N2 - The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

AB - The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

KW - A VIRUS

KW - MODEL RNA

KW - NONCODING REGIONS

KW - IN-VIVO

KW - EXPRESSION

KW - NEURAMINIDASE

KW - REPLICATION

KW - RESISTANCE

KW - POLYMERASE

KW - MUTATIONS

U2 - 10.1128/JVI.76.22.11744-11747.2002

DO - 10.1128/JVI.76.22.11744-11747.2002

M3 - Article

SN - 0022-538X

VL - 76

SP - 11744

EP - 11747

JO - Journal of Virology

JF - Journal of Virology

IS - 22

ER -

A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

Abstract

Keywords

UN SDGs

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