A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

David Jackson, A Cadman, T Zurcher, W S Barclay

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71 Citations (Scopus)

Abstract

The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

Original languageEnglish
Pages (from-to)11744-11747
Number of pages4
JournalJournal of Virology
Volume76
Issue number22
DOIs
Publication statusPublished - Nov 2002

Keywords

  • A VIRUS
  • MODEL RNA
  • NONCODING REGIONS
  • IN-VIVO
  • EXPRESSION
  • NEURAMINIDASE
  • REPLICATION
  • RESISTANCE
  • POLYMERASE
  • MUTATIONS

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