A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students

Grant R. Brown; Claire A. Dagen; Ben Reilly O'Donnell; Jeff A. Graves

doi:10.1080/00219266.2019.1620312

A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students

Grant R. Brown, Claire A. Dagen, Ben Reilly O'Donnell, Jeff A. Graves

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously naïve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with careful selection of specimens, undergraduate students could participate in sessions which both benefit their professional development and further taxonomic understanding across a variety of organisms.

Original language	English
Number of pages	9
Journal	Journal of Biological Education
Volume	Latest Articles
Early online date	23 May 2019
DOIs	https://doi.org/10.1080/00219266.2019.1620312
Publication status	E-pub ahead of print - 23 May 2019

Keywords

Education
Interdisciplinary
Taxonomy
Citizen science
Systematics

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10.1080/00219266.2019.1620312

Brown-2019-A-rapid-protocol-JBE-AAM
Copyright © 2019 Royal Society of Biology. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1080/00219266.2019.1620312
Accepted author manuscript, 186 KB
Brown_et_al_2018_Supplementary_InformationAccepted author manuscript, 702 KB

Cite this

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abstract = "We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously na{\"i}ve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with careful selection of specimens, undergraduate students could participate in sessions which both benefit their professional development and further taxonomic understanding across a variety of organisms.",

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KW - Interdisciplinary

KW - Taxonomy

KW - Citizen science

KW - Systematics

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A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students

Abstract

Keywords

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