Abstract
A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 299-305 |
| Number of pages | 7 |
| Journal | General and Comparative Endocrinology |
| Volume | 111 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sept 1998 |
Keywords
- ANGUILLA-ANGUILLA
- SYSTEM
- PLASMA
- EEL
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