A radioimmunoassay for the determination of angiotensin II in elasmobranch fish

ML Tierney, Y Takei, Neil Hazon

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume111
Issue number3
DOIs
Publication statusPublished - Sept 1998

Keywords

  • ANGUILLA-ANGUILLA
  • SYSTEM
  • PLASMA
  • EEL

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