A radioimmunoassay for the determination of angiotensin II in elasmobranch fish

ML Tierney; Y Takei; Neil Hazon

doi:10.1006/gcen.1998.7114

A radioimmunoassay for the determination of angiotensin II in elasmobranch fish

ML Tierney, Y Takei, Neil Hazon

Research output: Contribution to journal › Article › peer-review

Abstract

A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press.

Original language	English
Pages (from-to)	299-305
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	111
Issue number	3
DOIs	https://doi.org/10.1006/gcen.1998.7114
Publication status	Published - Sept 1998

Keywords

ANGUILLA-ANGUILLA
SYSTEM
PLASMA
EEL

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abstract = "A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press.",

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AU - Hazon, Neil

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AB - A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn(1), Pro(3), Ile(5))-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially crossreacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol ml(-1),respectively. (C) 1998 Academic Press.

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KW - SYSTEM

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KW - EEL

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A radioimmunoassay for the determination of angiotensin II in elasmobranch fish

Abstract

Keywords

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