A preliminary study into the use of tree-ring and foliar geochemistry as bio-indicators for vehicular NO_x pollution in Malta

Emissions from traffic over the past few decades have become a significant source of air pollution. Among the pollutants emitted are nitrogen oxides (NO_x), exposure to which can be detrimental to public health. Recent studies have shown that nitrogen (N) stable isotope ratios in tree-rings and foliage express a fingerprint of their major N source, making them appropriate for bio-monitoring purposes. In this study, we have applied this proxy to Aleppo pines (Pinus halepensis) at three distances from one of the busiest roads in Malta, a country known to suffer from intense traffic pollution. Our results showed that N and organic carbon (C) stable isotope ratios in tree-rings do not vary over the period 1980–2018 at any of the investigated sites; however, statistically significant spatial trends were apparent in both tree-rings and foliage. The roadside and transitional sites exhibited more positive δ¹⁵N and more negative δ¹³C values compared to those at a rural control site. This is likely due to the incorporation of ¹⁵N-enriched NO_x and ¹³C-depleted CO₂ from traffic pollution. Sampled top-soil also exhibited the δ¹⁵N trend. Our results constitute the first known application of dendrogeochemistry to atmospheric pollution monitoring in Malta.