A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory

K. J. Williams; C. L. Ling; C. Jenkins; S. H. Gillespie; T. D. McHugh

doi:10.1099/jmm.0.46855-0

A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory

K. J. Williams, C. L. Ling, C. Jenkins, S. H. Gillespie, T. D. McHugh

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S rRNA gene sequencing for the speciation of MOTT. Real-time PCR assays gave comparable results to those from the reference laboratory. The implementation of these PCR assays using an improved bead extraction method has enhanced the mycobacterial diagnostic service at the Royal Free Hospital by providing a rapid means of differentiating between MTB complex and MOTT, and would be simple to implement in similar laboratories. Sequence analysis successfully identified a range of Mycobacterium spp. representative of those encountered in the clinical setting of the authors, including Mycobacterium avium complex, Mycobacterium fortuitum group, Mycobacterium chelonae-Mycobacterium abscessus group, Mycobacterium xenopi and Mycobacterium gordonae. It provides a useful tool for the identification of MOTT when clinically indicated.

Original language	English
Pages (from-to)	598-602
Number of pages	5
Journal	Journal of Medical Microbiology
Volume	56
Issue number	5
DOIs	https://doi.org/10.1099/jmm.0.46855-0
Publication status	Published - May 2007

Keywords

RAPIDLY GROWING MYCOBACTERIA
RIBOSOMAL-RNA GENE
FRAGMENT-LENGTH-POLYMORPHISM
CAPTURE PLATE HYBRIDIZATION
CHAIN-REACTION
NONTUBERCULOUS MYCOBACTERIA
TUBERCULOSIS COMPLEX
DNA AMPLIFICATION
PCR ASSAY
RPOB

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1099/jmm.0.46855-0

Cite this

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title = "A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory",

abstract = "The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S rRNA gene sequencing for the speciation of MOTT. Real-time PCR assays gave comparable results to those from the reference laboratory. The implementation of these PCR assays using an improved bead extraction method has enhanced the mycobacterial diagnostic service at the Royal Free Hospital by providing a rapid means of differentiating between MTB complex and MOTT, and would be simple to implement in similar laboratories. Sequence analysis successfully identified a range of Mycobacterium spp. representative of those encountered in the clinical setting of the authors, including Mycobacterium avium complex, Mycobacterium fortuitum group, Mycobacterium chelonae-Mycobacterium abscessus group, Mycobacterium xenopi and Mycobacterium gordonae. It provides a useful tool for the identification of MOTT when clinically indicated.",

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author = "Williams, {K. J.} and Ling, {C. L.} and C. Jenkins and Gillespie, {S. H.} and McHugh, {T. D.}",

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doi = "10.1099/jmm.0.46855-0",

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AU - Williams, K. J.

AU - Ling, C. L.

AU - Jenkins, C.

AU - Gillespie, S. H.

AU - McHugh, T. D.

PY - 2007/5

Y1 - 2007/5

N2 - The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S rRNA gene sequencing for the speciation of MOTT. Real-time PCR assays gave comparable results to those from the reference laboratory. The implementation of these PCR assays using an improved bead extraction method has enhanced the mycobacterial diagnostic service at the Royal Free Hospital by providing a rapid means of differentiating between MTB complex and MOTT, and would be simple to implement in similar laboratories. Sequence analysis successfully identified a range of Mycobacterium spp. representative of those encountered in the clinical setting of the authors, including Mycobacterium avium complex, Mycobacterium fortuitum group, Mycobacterium chelonae-Mycobacterium abscessus group, Mycobacterium xenopi and Mycobacterium gordonae. It provides a useful tool for the identification of MOTT when clinically indicated.

AB - The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S rRNA gene sequencing for the speciation of MOTT. Real-time PCR assays gave comparable results to those from the reference laboratory. The implementation of these PCR assays using an improved bead extraction method has enhanced the mycobacterial diagnostic service at the Royal Free Hospital by providing a rapid means of differentiating between MTB complex and MOTT, and would be simple to implement in similar laboratories. Sequence analysis successfully identified a range of Mycobacterium spp. representative of those encountered in the clinical setting of the authors, including Mycobacterium avium complex, Mycobacterium fortuitum group, Mycobacterium chelonae-Mycobacterium abscessus group, Mycobacterium xenopi and Mycobacterium gordonae. It provides a useful tool for the identification of MOTT when clinically indicated.

KW - RAPIDLY GROWING MYCOBACTERIA

KW - RIBOSOMAL-RNA GENE

KW - FRAGMENT-LENGTH-POLYMORPHISM

KW - CAPTURE PLATE HYBRIDIZATION

KW - CHAIN-REACTION

KW - NONTUBERCULOUS MYCOBACTERIA

KW - TUBERCULOSIS COMPLEX

KW - DNA AMPLIFICATION

KW - PCR ASSAY

KW - RPOB

U2 - 10.1099/jmm.0.46855-0

DO - 10.1099/jmm.0.46855-0

M3 - Article

SN - 0022-2615

VL - 56

SP - 598

EP - 602

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

IS - 5

ER -

A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory

Abstract

Keywords

UN SDGs

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