A new facile method for spin-labeling of oligonucleotides

T. Strube, Olav Schiemann, F. MacMillan, T.F. Prisner, J.W. Engels

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

Original languageEnglish
Pages (from-to)1271-1274
Number of pages4
JournalNucleosides, Nucleotides and Nucleic Acids
Volume20
Issue number4-7
DOIs
Publication statusPublished - Mar 2001

Keywords

  • DNA
  • DUPLEX
  • PROBE
  • SITE

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