A new facile method for spin-labeling of oligonucleotides

T. Strube; Olav Schiemann; F. MacMillan; T.F. Prisner; J.W. Engels

doi:10.1081/NCN-100002534

A new facile method for spin-labeling of oligonucleotides

T. Strube, Olav Schiemann, F. MacMillan, T.F. Prisner, J.W. Engels

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

Original language	English
Pages (from-to)	1271-1274
Number of pages	4
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	20
Issue number	4-7
DOIs	https://doi.org/10.1081/NCN-100002534
Publication status	Published - Mar 2001

Keywords

DNA
DUPLEX
PROBE
SITE

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10.1081/NCN-100002534

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AU - Schiemann, Olav

AU - MacMillan, F.

AU - Prisner, T.F.

AU - Engels, J.W.

PY - 2001/3

Y1 - 2001/3

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AB - A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

KW - DNA

KW - DUPLEX

KW - PROBE

KW - SITE

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JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 4-7

ER -

A new facile method for spin-labeling of oligonucleotides

Abstract

Keywords

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