A Multidisciplinary Approach to Probing Enthalpy-Entropy Compensation and the Interfacial Mobility Model

Erin M. Wilfong, Yuri Kogiso, Sivaramakrishnan Muthukrishnan, Thomas Kowatz, Yu Du, Amber Bowie, James H. Naismith, Christopher M. Hadad, Eric J. Toone, Terry L. Gustafson

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In recent years, interfacial mobility has gained popularity as a model with which to rationalize both affinity in ligand binding and the often observed phenomenon of enthalpy-entropy compensation. While protein contraction and reduced mobility, as demonstrated by computational and NMR techniques respectively, have been correlated to entropies of binding for a variety of systems, to our knowledge, Raman difference spectroscopy has never been included in these analyses. Here, nonresonance Raman difference spectroscopy, isothermal titration calorimetry, and X-ray crystallography were utilized to correlate protein contraction, as demonstrated by an increase in protein interior packing and decreased residual protein movement, with trends of enthalpy-entropy compensation. These results are in accord with the interfacial mobility model and lend additional credence to this view of protein activity.

Original languageEnglish
Pages (from-to)11515-11523
Number of pages9
JournalJournal of the American Chemical Society
Volume133
Issue number30
DOIs
Publication statusPublished - 3 Aug 2011

Keywords

  • STROMELYSIN CATALYTIC DOMAIN
  • X-RAY-STRUCTURE
  • MATRIX-METALLOPROTEINASE INHIBITORS
  • RAMAN DIFFERENCE SPECTROSCOPY
  • TRYPTOPHAN SIDE-CHAINS
  • LIGAND-BINDING ENERGY
  • CARBONIC-ANHYDRASE-II
  • PROTEIN-STRUCTURE
  • SOLVENT REORGANIZATION
  • CONFORMATIONAL ENTROPY

Fingerprint

Dive into the research topics of 'A Multidisciplinary Approach to Probing Enthalpy-Entropy Compensation and the Interfacial Mobility Model'. Together they form a unique fingerprint.

Cite this