A molecular mechanism for modulating plasma Zn speciation by fatty acids

Jin Lu; Alan J. Stewart; Darrell Sleep; Peter J. Sadler; Teresa J. T. Pinheiro; Claudia A. Blindauer

doi:10.1021/ja210496n

A molecular mechanism for modulating plasma Zn speciation by fatty acids

Jin Lu, Alan J. Stewart, Darrell Sleep, Peter J. Sadler, Teresa J. T. Pinheiro, Claudia A. Blindauer

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Albumin transports both fatty acids and zinc in plasma. Competitive binding studied by isothermal titration calorimetry revealed that physiologically relevant levels of fatty acids modulate the Zn-binding capacity of albumin, with far-reaching implications for biological zinc speciation. The molecular mechanism for this effect is likely due to a large conformational change elicited by fatty acid binding to an high-affinity inter-domain site that disrupts at least one Zn site. Albumin may be a molecular device to "translate" certain aspects of the organismal energy state into global zinc signals.

Original language	English
Pages (from-to)	1454-1457
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	134
Issue number	3
Early online date	4 Jan 2012
DOIs	https://doi.org/10.1021/ja210496n
Publication status	Published - 25 Jan 2012

Access to Document

10.1021/ja210496n

Cite this

@article{bf79422a34f04cf19aca560482684897,

title = "A molecular mechanism for modulating plasma Zn speciation by fatty acids",

abstract = "Albumin transports both fatty acids and zinc in plasma. Competitive binding studied by isothermal titration calorimetry revealed that physiologically relevant levels of fatty acids modulate the Zn-binding capacity of albumin, with far-reaching implications for biological zinc speciation. The molecular mechanism for this effect is likely due to a large conformational change elicited by fatty acid binding to an high-affinity inter-domain site that disrupts at least one Zn site. Albumin may be a molecular device to {"}translate{"} certain aspects of the organismal energy state into global zinc signals. ",

author = "Jin Lu and Stewart, {Alan J.} and Darrell Sleep and Sadler, {Peter J.} and Pinheiro, {Teresa J. T.} and Blindauer, {Claudia A.}",

year = "2012",

month = jan,

day = "25",

doi = "10.1021/ja210496n",

language = "English",

volume = "134",

pages = "1454--1457",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society (ACS)",

number = "3",

}

TY - JOUR

T1 - A molecular mechanism for modulating plasma Zn speciation by fatty acids

AU - Lu, Jin

AU - Stewart, Alan J.

AU - Sleep, Darrell

AU - Sadler, Peter J.

AU - Pinheiro, Teresa J. T.

AU - Blindauer, Claudia A.

PY - 2012/1/25

Y1 - 2012/1/25

N2 - Albumin transports both fatty acids and zinc in plasma. Competitive binding studied by isothermal titration calorimetry revealed that physiologically relevant levels of fatty acids modulate the Zn-binding capacity of albumin, with far-reaching implications for biological zinc speciation. The molecular mechanism for this effect is likely due to a large conformational change elicited by fatty acid binding to an high-affinity inter-domain site that disrupts at least one Zn site. Albumin may be a molecular device to "translate" certain aspects of the organismal energy state into global zinc signals.

AB - Albumin transports both fatty acids and zinc in plasma. Competitive binding studied by isothermal titration calorimetry revealed that physiologically relevant levels of fatty acids modulate the Zn-binding capacity of albumin, with far-reaching implications for biological zinc speciation. The molecular mechanism for this effect is likely due to a large conformational change elicited by fatty acid binding to an high-affinity inter-domain site that disrupts at least one Zn site. Albumin may be a molecular device to "translate" certain aspects of the organismal energy state into global zinc signals.

UR - http://ukpmc.ac.uk/articles/PMC3285120

UR - http://pubs.acs.org/doi/pdf/10.1021/ja210496n

U2 - 10.1021/ja210496n

DO - 10.1021/ja210496n

M3 - Article

SN - 0002-7863

VL - 134

SP - 1454

EP - 1457

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 3

ER -

A molecular mechanism for modulating plasma Zn speciation by fatty acids

Abstract

Access to Document

Other files and links

Fingerprint

Cite this