A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells

Reza Abazari; Farangis Ataei; Ali Morsali; Alexandra M. Z. Slawin; Cameron L. Carpenter-Warren

doi:10.1021/acsami.9b16473

A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells

Reza Abazari
, Farangis Ataei
, Ali Morsali^*
, Alexandra M. Z. Slawin
, Cameron L. Carpenter-Warren

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Folic acid amine-functionalized metal-organic framework (FOLA@NH2-Eu:TMU-62) with luminescent properties loaded with 5-fluorouracil (5-Fu), as an anticancer medication, was used to construct a new cancer targeted drug delivery system in the present study. The 5-Fu release from this targeted carrier along with MTT assay and trypan blue dye exclusion test results also exhibited pH-controlled characteristics of the given carrier in acidic environments, which is very suitable for targeting solid tumors. Then, the inhibitory action of 5-Fu-loaded FOLA@NH2-Eu:TMU-62 for Michigan Cancer Foundation-7 (MCF7) cell migration was explored according to scratch wound healing assays. Based on the results, the FOLA@NH2-Eu:TMU-62 carrier was not toxic for MCF-10A normal cells, but it was significantly toxic for MCF-7 breast cancer ones, revealing that the FOLA@NH2-Eu:TMU-62 carrier could be utilized in accurate cancer treatments through apoptotic pathways with higher reactive oxygen species compared with 5-Fu alone. This cancer-targeted design of FOLA@NH2-Eu:TMU-62 could thus pave the way for synergistic effects of targeting as well as organized release capabilities.

Original language	English
Pages (from-to)	45442-45454
Number of pages	13
Journal	ACS Applied Materials & Interfaces
Volume	11
Issue number	49
Early online date	13 Nov 2019
DOIs	https://doi.org/10.1021/acsami.9b16473
Publication status	Published - 11 Dec 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

metal-organic framework
targeted design
pH-responsive nanocarrier
breast cancer cells
wound healing
ONE-POT SYNTHESIS
DRUG-DELIVERY
IN-VITRO
NANOPARTICLES
THERAPY
DESIGN

Access to Document

10.1021/acsami.9b16473

Handle.net

Persistent link to portal record

A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells (dataset)
Abazari, R. (Creator), Ataei, F. (Creator), Morsali, A. (Creator), Slawin, A. M. Z. (Creator) & Carpenter-Warren, C. L. (Creator), Figshare, 2019
https://figshare.com/articles/A_Luminescent_Amine-Functionalized_Metal_Organic_Framework_Conjugated_with_Folic_Acid_as_a_Targeted_Biocompatible_pH-Responsive_Nanocarrier_for_Apoptosis_Induction_in_Breast_Cancer_Cells/11116880
Dataset

Cite this

Abazari, R., Ataei, F., Morsali, A., Slawin, A. M. Z., & Carpenter-Warren, C. L. (2019). A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells. ACS Applied Materials & Interfaces, 11(49), 45442-45454. https://doi.org/10.1021/acsami.9b16473

@article{8dffabe5a8834c0f9054296bffb7e617,

title = "A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells",

abstract = "Folic acid amine-functionalized metal-organic framework (FOLA@NH2-Eu:TMU-62) with luminescent properties loaded with 5-fluorouracil (5-Fu), as an anticancer medication, was used to construct a new cancer targeted drug delivery system in the present study. The 5-Fu release from this targeted carrier along with MTT assay and trypan blue dye exclusion test results also exhibited pH-controlled characteristics of the given carrier in acidic environments, which is very suitable for targeting solid tumors. Then, the inhibitory action of 5-Fu-loaded FOLA@NH2-Eu:TMU-62 for Michigan Cancer Foundation-7 (MCF7) cell migration was explored according to scratch wound healing assays. Based on the results, the FOLA@NH2-Eu:TMU-62 carrier was not toxic for MCF-10A normal cells, but it was significantly toxic for MCF-7 breast cancer ones, revealing that the FOLA@NH2-Eu:TMU-62 carrier could be utilized in accurate cancer treatments through apoptotic pathways with higher reactive oxygen species compared with 5-Fu alone. This cancer-targeted design of FOLA@NH2-Eu:TMU-62 could thus pave the way for synergistic effects of targeting as well as organized release capabilities.",

keywords = "metal-organic framework, targeted design, pH-responsive nanocarrier, breast cancer cells, wound healing, ONE-POT SYNTHESIS, DRUG-DELIVERY, IN-VITRO, NANOPARTICLES, THERAPY, DESIGN",

author = "Reza Abazari and Farangis Ataei and Ali Morsali and Slawin, \{Alexandra M. Z.\} and Carpenter-Warren, \{Cameron L.\}",

year = "2019",

month = dec,

day = "11",

doi = "10.1021/acsami.9b16473",

language = "English",

volume = "11",

pages = "45442--45454",

journal = "ACS Applied Materials \& Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society (ACS)",

number = "49",

}

Abazari, R, Ataei, F, Morsali, A, Slawin, AMZ & Carpenter-Warren, CL 2019, 'A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells', ACS Applied Materials & Interfaces, vol. 11, no. 49, pp. 45442-45454. https://doi.org/10.1021/acsami.9b16473

A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells. / Abazari, Reza; Ataei, Farangis; Morsali, Ali et al.
In: ACS Applied Materials & Interfaces, Vol. 11, No. 49, 11.12.2019, p. 45442-45454.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells

AU - Abazari, Reza

AU - Ataei, Farangis

AU - Morsali, Ali

AU - Slawin, Alexandra M. Z.

AU - Carpenter-Warren, Cameron L.

PY - 2019/12/11

Y1 - 2019/12/11

N2 - Folic acid amine-functionalized metal-organic framework (FOLA@NH2-Eu:TMU-62) with luminescent properties loaded with 5-fluorouracil (5-Fu), as an anticancer medication, was used to construct a new cancer targeted drug delivery system in the present study. The 5-Fu release from this targeted carrier along with MTT assay and trypan blue dye exclusion test results also exhibited pH-controlled characteristics of the given carrier in acidic environments, which is very suitable for targeting solid tumors. Then, the inhibitory action of 5-Fu-loaded FOLA@NH2-Eu:TMU-62 for Michigan Cancer Foundation-7 (MCF7) cell migration was explored according to scratch wound healing assays. Based on the results, the FOLA@NH2-Eu:TMU-62 carrier was not toxic for MCF-10A normal cells, but it was significantly toxic for MCF-7 breast cancer ones, revealing that the FOLA@NH2-Eu:TMU-62 carrier could be utilized in accurate cancer treatments through apoptotic pathways with higher reactive oxygen species compared with 5-Fu alone. This cancer-targeted design of FOLA@NH2-Eu:TMU-62 could thus pave the way for synergistic effects of targeting as well as organized release capabilities.

AB - Folic acid amine-functionalized metal-organic framework (FOLA@NH2-Eu:TMU-62) with luminescent properties loaded with 5-fluorouracil (5-Fu), as an anticancer medication, was used to construct a new cancer targeted drug delivery system in the present study. The 5-Fu release from this targeted carrier along with MTT assay and trypan blue dye exclusion test results also exhibited pH-controlled characteristics of the given carrier in acidic environments, which is very suitable for targeting solid tumors. Then, the inhibitory action of 5-Fu-loaded FOLA@NH2-Eu:TMU-62 for Michigan Cancer Foundation-7 (MCF7) cell migration was explored according to scratch wound healing assays. Based on the results, the FOLA@NH2-Eu:TMU-62 carrier was not toxic for MCF-10A normal cells, but it was significantly toxic for MCF-7 breast cancer ones, revealing that the FOLA@NH2-Eu:TMU-62 carrier could be utilized in accurate cancer treatments through apoptotic pathways with higher reactive oxygen species compared with 5-Fu alone. This cancer-targeted design of FOLA@NH2-Eu:TMU-62 could thus pave the way for synergistic effects of targeting as well as organized release capabilities.

KW - metal-organic framework

KW - targeted design

KW - pH-responsive nanocarrier

KW - breast cancer cells

KW - wound healing

KW - ONE-POT SYNTHESIS

KW - DRUG-DELIVERY

KW - IN-VITRO

KW - NANOPARTICLES

KW - THERAPY

KW - DESIGN

U2 - 10.1021/acsami.9b16473

DO - 10.1021/acsami.9b16473

M3 - Article

SN - 1944-8244

VL - 11

SP - 45442

EP - 45454

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

IS - 49

ER -

A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells

Abstract

UN SDGs

Keywords

Access to Document

Handle.net

Fingerprint

Datasets

A Luminescent Amine-Functionalized Metal-Organic Framework Conjugated with Folic Acid as a Targeted Biocompatible pH-Responsive Nanocarrier for Apoptosis Induction in Breast Cancer Cells (dataset)

Cite this