A high-throughput screening of a chemical compound library in ovarian cancer stem cells

F. Ricci; L. Carrassa; M. S. Christodoulou; D. Passarella; B. Michel; B. Benhida; N. Martinet; A. Hunyadi; E. Ioannou; V. Roussis; L. Musso; S. Dallavalle; R. Silvestri; Nicholas James Westwood; M. Mori; C. Ingallina; B. Botta; E. Kavetsou; A. Detsi; Z. Majer; F. Hudecz; S. Bősze; B. Kaminska; T. V. Hansen; P. Bertrand; C. M. Athanassopoulos; G. Damia

doi:10.2174/1386207321666180124093406

A high-throughput screening of a chemical compound library in ovarian cancer stem cells

F. Ricci, L. Carrassa, M. S. Christodoulou, D. Passarella, B. Michel, B. Benhida, N. Martinet , A. Hunyadi, E. Ioannou, V. Roussis, L. Musso, S. Dallavalle , R. Silvestri, Nicholas James Westwood, M. Mori, C. Ingallina, B. Botta, E. Kavetsou, A. Detsi, Z. MajerF. Hudecz, S. Bősze, B. Kaminska, T. V. Hansen, P. Bertrand, C. M. Athanassopoulos, G. Damia

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Epithelial ovarian cancer has a poor prognosis, mostly due to its late diagnosis and to the development of drug resistance after a first platinum-based regimen. The presence of a specific population of “cancer stem cells” could be responsible of the relapse of the tumor, and of the development of resistance to therapy. For this reason, it would be important to specifically target this subpopulation of tumor cells in order to increase the response to therapy.
Method: We screened a chemical compound library assembled during the COST CM1106 action to search for compound classes active in targeting ovarian stem cells. We here report the results of the high-throughput screening assay in two ovarian cancer stem cells and the differentiated cells derived from them.
Results and conclusion: Interestingly there were compounds active only on stem cells, only on differentiated cells and compounds active on both cell populations. Even if these data need to be validated in ad hoc dose response cytotoxic experiments, the ongoing analysis of the compound structures will open up to mechanistic drug studies to select compounds able to improve the prognosis of ovarian cancer patients.

Original language	English
Pages (from-to)	50-56
Journal	Combinatorial Chemistry and High Throughput Screening
Volume	21
Issue number	1
Early online date	23 Jan 2018
DOIs	https://doi.org/10.2174/1386207321666180124093406
Publication status	E-pub ahead of print - 23 Jan 2018

Keywords

Cancer stem cell
Chemical compounds library
Oncology screening
High-throughput screening
Ovarian cancer
Therapy resistance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2174/1386207321666180124093406

Westwood_2018_CCHTS_HighthroughputCemComLibOvCancer_AAM
© 2018 Bentham Science Publishers. This work has been made available online in accordance with the publisher’s policies. This is the author created accepted version manuscript following peer review and as such may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.2174/1386207321666180124093406
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Ricci, F., Carrassa, L., Christodoulou, M. S., Passarella, D., Michel, B., Benhida, B., Martinet , N., Hunyadi, A., Ioannou, E., Roussis, V., Musso, L., Dallavalle , S., Silvestri, R., Westwood, N. J., Mori, M., Ingallina, C., Botta, B., Kavetsou, E., Detsi, A., ... Damia, G. (2018). A high-throughput screening of a chemical compound library in ovarian cancer stem cells. Combinatorial Chemistry and High Throughput Screening , 21(1), 50-56. Advance online publication. https://doi.org/10.2174/1386207321666180124093406

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title = "A high-throughput screening of a chemical compound library in ovarian cancer stem cells",

abstract = "Background: Epithelial ovarian cancer has a poor prognosis, mostly due to its late diagnosis and to the development of drug resistance after a first platinum-based regimen. The presence of a specific population of “cancer stem cells” could be responsible of the relapse of the tumor, and of the development of resistance to therapy. For this reason, it would be important to specifically target this subpopulation of tumor cells in order to increase the response to therapy. Method: We screened a chemical compound library assembled during the COST CM1106 action to search for compound classes active in targeting ovarian stem cells. We here report the results of the high-throughput screening assay in two ovarian cancer stem cells and the differentiated cells derived from them.Results and conclusion: Interestingly there were compounds active only on stem cells, only on differentiated cells and compounds active on both cell populations. Even if these data need to be validated in ad hoc dose response cytotoxic experiments, the ongoing analysis of the compound structures will open up to mechanistic drug studies to select compounds able to improve the prognosis of ovarian cancer patients.",

keywords = "Cancer stem cell, Chemical compounds library, Oncology screening, High-throughput screening, Ovarian cancer, Therapy resistance",

author = "F. Ricci and L. Carrassa and Christodoulou, {M. S.} and D. Passarella and B. Michel and B. Benhida and N. Martinet and A. Hunyadi and E. Ioannou and V. Roussis and L. Musso and S. Dallavalle and R. Silvestri and Westwood, {Nicholas James} and M. Mori and C. Ingallina and B. Botta and E. Kavetsou and A. Detsi and Z. Majer and F. Hudecz and S. B{\H o}sze and B. Kaminska and Hansen, {T. V.} and P. Bertrand and Athanassopoulos, {C. M.} and G. Damia",

note = "This work was performed under the frame of COST Action collaboration (COST Action CM1106). The generous contribution of AIRC (The Italian Association for Cancer Research) IG14536 to G.D. is gratefully acknowledged. A.H. acknowledges support from the J{\'a}nos Bolyai fellowship of the Hungarian Academy of Sciences. ",

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doi = "10.2174/1386207321666180124093406",

language = "English",

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Ricci, F, Carrassa, L, Christodoulou, MS, Passarella, D, Michel, B, Benhida, B, Martinet , N, Hunyadi, A, Ioannou, E, Roussis, V, Musso, L, Dallavalle , S, Silvestri, R, Westwood, NJ, Mori, M, Ingallina, C, Botta, B, Kavetsou, E, Detsi, A, Majer, Z, Hudecz, F, Bősze, S, Kaminska, B, Hansen, TV, Bertrand, P, Athanassopoulos, CM & Damia, G 2018, 'A high-throughput screening of a chemical compound library in ovarian cancer stem cells', Combinatorial Chemistry and High Throughput Screening , vol. 21, no. 1, pp. 50-56. https://doi.org/10.2174/1386207321666180124093406

TY - JOUR

T1 - A high-throughput screening of a chemical compound library in ovarian cancer stem cells

AU - Ricci, F.

AU - Carrassa, L.

AU - Christodoulou, M. S.

AU - Passarella, D.

AU - Michel, B.

AU - Benhida, B.

AU - Martinet , N.

AU - Hunyadi, A.

AU - Ioannou, E.

AU - Roussis, V.

AU - Musso, L.

AU - Dallavalle , S.

AU - Silvestri, R.

AU - Westwood, Nicholas James

AU - Mori, M.

AU - Ingallina, C.

AU - Botta, B.

AU - Kavetsou, E.

AU - Detsi, A.

AU - Majer, Z.

AU - Hudecz, F.

AU - Bősze, S.

AU - Kaminska, B.

AU - Hansen, T. V.

AU - Bertrand, P.

AU - Athanassopoulos, C. M.

AU - Damia, G.

N1 - This work was performed under the frame of COST Action collaboration (COST Action CM1106). The generous contribution of AIRC (The Italian Association for Cancer Research) IG14536 to G.D. is gratefully acknowledged. A.H. acknowledges support from the János Bolyai fellowship of the Hungarian Academy of Sciences.

PY - 2018/1/23

Y1 - 2018/1/23

N2 - Background: Epithelial ovarian cancer has a poor prognosis, mostly due to its late diagnosis and to the development of drug resistance after a first platinum-based regimen. The presence of a specific population of “cancer stem cells” could be responsible of the relapse of the tumor, and of the development of resistance to therapy. For this reason, it would be important to specifically target this subpopulation of tumor cells in order to increase the response to therapy. Method: We screened a chemical compound library assembled during the COST CM1106 action to search for compound classes active in targeting ovarian stem cells. We here report the results of the high-throughput screening assay in two ovarian cancer stem cells and the differentiated cells derived from them.Results and conclusion: Interestingly there were compounds active only on stem cells, only on differentiated cells and compounds active on both cell populations. Even if these data need to be validated in ad hoc dose response cytotoxic experiments, the ongoing analysis of the compound structures will open up to mechanistic drug studies to select compounds able to improve the prognosis of ovarian cancer patients.

AB - Background: Epithelial ovarian cancer has a poor prognosis, mostly due to its late diagnosis and to the development of drug resistance after a first platinum-based regimen. The presence of a specific population of “cancer stem cells” could be responsible of the relapse of the tumor, and of the development of resistance to therapy. For this reason, it would be important to specifically target this subpopulation of tumor cells in order to increase the response to therapy. Method: We screened a chemical compound library assembled during the COST CM1106 action to search for compound classes active in targeting ovarian stem cells. We here report the results of the high-throughput screening assay in two ovarian cancer stem cells and the differentiated cells derived from them.Results and conclusion: Interestingly there were compounds active only on stem cells, only on differentiated cells and compounds active on both cell populations. Even if these data need to be validated in ad hoc dose response cytotoxic experiments, the ongoing analysis of the compound structures will open up to mechanistic drug studies to select compounds able to improve the prognosis of ovarian cancer patients.

KW - Cancer stem cell

KW - Chemical compounds library

KW - Oncology screening

KW - High-throughput screening

KW - Ovarian cancer

KW - Therapy resistance

U2 - 10.2174/1386207321666180124093406

DO - 10.2174/1386207321666180124093406

M3 - Article

SN - 1386-2073

VL - 21

SP - 50

EP - 56

JO - Combinatorial Chemistry and High Throughput Screening

JF - Combinatorial Chemistry and High Throughput Screening

IS - 1

ER -

A high-throughput screening of a chemical compound library in ovarian cancer stem cells

Abstract

Keywords

UN SDGs

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