A comparison of the efficiency of differential display and cDNA-AFLPs as tools for the isolation of differentially expressed parasite genes

JT Jones*, BE Harrower

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A range of novel PCR-based techniques for the isolation of differentially expressed genes have recently been described for the isolation of stage-specific or host-responsive parasite genes. We-have been using two such techniques, differential display and cDNA-AFLPs, in studies on gene expression changes during hatching and subsequent development of the potato cyst nematodes Globodera rostochiensis and G. pallida. In this paper, we compare the efficiency of these two techniques for the isolation of such genes. cDNA-AFLPs were found to be an extremely efficient method of isolating differentially expressed parasite genes while, in contrast, differential display was of limited value. Problems associated with the techniques and their use with plant parasitic nematodes are discussed.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalFundamental and Applied Nematology
Volume21
Issue number1
Publication statusPublished - 1998

Keywords

  • cDNA-AFLP
  • differential display
  • gene expression
  • potato cyst nematode
  • RNA

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