Abstract
A range of novel PCR-based techniques for the isolation of differentially expressed genes have recently been described for the isolation of stage-specific or host-responsive parasite genes. We-have been using two such techniques, differential display and cDNA-AFLPs, in studies on gene expression changes during hatching and subsequent development of the potato cyst nematodes Globodera rostochiensis and G. pallida. In this paper, we compare the efficiency of these two techniques for the isolation of such genes. cDNA-AFLPs were found to be an extremely efficient method of isolating differentially expressed parasite genes while, in contrast, differential display was of limited value. Problems associated with the techniques and their use with plant parasitic nematodes are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 81-88 |
| Number of pages | 8 |
| Journal | Fundamental and Applied Nematology |
| Volume | 21 |
| Issue number | 1 |
| Publication status | Published - 1998 |
Keywords
- cDNA-AFLP
- differential display
- gene expression
- potato cyst nematode
- RNA