A compact light-sheet microscope for the study of the mammalian central nervous system

Zhengyi Yang; Peter Haslehurst; Suzanne Scott; Nigel Emptage; Kishan Dholakia

doi:10.1038/srep26317

A compact light-sheet microscope for the study of the mammalian central nervous system

Zhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45^◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca²⁺ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

Original language	English
Article number	26317
Number of pages	7
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep26317
Publication status	Published - 24 May 2016

Access to Document

10.1038/srep26317Licence: CC BY

Yang_2016_ACompact_SREP_CC
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Final published version, 871 KBLicence: CC BY

http://www.nature.com/articles/srep26317Licence: CC BY

Cite this

@article{2f781e802de44d299ede7b3dded1ac31,

title = "A compact light-sheet microscope for the study of the mammalian central nervous system",

abstract = "Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.",

author = "Zhengyi Yang and Peter Haslehurst and Suzanne Scott and Nigel Emptage and Kishan Dholakia",

note = "We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.",

year = "2016",

month = may,

day = "24",

doi = "10.1038/srep26317",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature publishing group",

}

TY - JOUR

T1 - A compact light-sheet microscope for the study of the mammalian central nervous system

AU - Yang, Zhengyi

AU - Haslehurst, Peter

AU - Scott, Suzanne

AU - Emptage, Nigel

AU - Dholakia, Kishan

N1 - We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.

PY - 2016/5/24

Y1 - 2016/5/24

N2 - Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

AB - Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

UR - https://www.scopus.com/pages/publications/84971351690

U2 - 10.1038/srep26317

DO - 10.1038/srep26317

M3 - Article

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 26317

ER -

A compact light-sheet microscope for the study of the mammalian central nervous system

Abstract

Access to Document

Other files and links

Fingerprint

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

Data underpinning - A compact light-sheet microscope for the study of the mammalian central nervous system

Cite this

A compact light-sheet microscope for the study of the mammalian central nervous system

Abstract

Access to Document

Other files and links

Fingerprint

Projects

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

Datasets

Data underpinning - A compact light-sheet microscope for the study of the mammalian central nervous system

Cite this