A compact light-sheet microscope for the study of the mammalian central nervous system

Zhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45 inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
Original languageEnglish
Article number26317
Number of pages7
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 24 May 2016

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