A compact Airy beam light sheet microscope with a tilted cylindrical lens

Zhengyi Yang; Martynas Prokopas; Jonathan Nylk; Clara Coll Llado; Frank J Gunn-Moore; David Ellard Keith Ferrier; Tom Vettenburg; Kishan Dholakia

doi:10.1364/BOE.5.003434

A compact Airy beam light sheet microscope with a tilted cylindrical lens

Zhengyi Yang, Martynas Prokopas, Jonathan Nylk, Clara Coll Llado, Frank J Gunn-Moore, David Ellard Keith Ferrier, Tom Vettenburg, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.

Original language	English
Pages (from-to)	3434-3442
Journal	Biomedical Optics Express
Volume	5
Issue number	10
Early online date	5 Sept 2014
DOIs	https://doi.org/10.1364/BOE.5.003434
Publication status	Published - 1 Oct 2014

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10.1364/BOE.5.003434

boe-5-10-3434
(c) Copyright 2014 Optical Society of America. This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: [http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-10-3434]. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
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Cite this

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title = "A compact Airy beam light sheet microscope with a tilted cylindrical lens",

abstract = "Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.",

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doi = "10.1364/BOE.5.003434",

language = "English",

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AU - Yang, Zhengyi

AU - Prokopas, Martynas

AU - Nylk, Jonathan

AU - Coll Llado, Clara

AU - Gunn-Moore, Frank J

AU - Ferrier, David Ellard Keith

AU - Vettenburg, Tom

AU - Dholakia, Kishan

N1 - We thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal and Dr. E. Killick for funding.

PY - 2014/10/1

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N2 - Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.

AB - Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.

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JF - Biomedical Optics Express

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A compact Airy beam light sheet microscope with a tilted cylindrical lens

Abstract

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Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

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A compact Airy beam light sheet microscope with a tilted cylindrical lens

Abstract

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Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

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Evolution of muscle regulatory genes in chordates

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