A cassette vector for the construction of antigen chimaeras of poliovirus

K L Burke; D J Evans; O Jenkins; J Meredith; E D D'Souza; J W Almond

A cassette vector for the construction of antigen chimaeras of poliovirus

K L Burke, D J Evans, O Jenkins, J Meredith, E D D'Souza, J W Almond

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.

Original language	English
Pages (from-to)	2475-9
Number of pages	5
Journal	Journal of General Virology
Volume	70 ( Pt 9)
Publication status	Published - Sept 1989

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Amino Acid Sequence
Antigens, Viral
Base Sequence
Chimera
DNA, Viral
Epitopes
Genes, Viral
Genetic Vectors
Molecular Sequence Data
Neutralization Tests
Poliovirus
Poliovirus Vaccine, Oral
Restriction Mapping

Cite this

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title = "A cassette vector for the construction of antigen chimaeras of poliovirus",

abstract = "A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.",

keywords = "Amino Acid Sequence, Antigens, Viral, Base Sequence, Chimera, DNA, Viral, Epitopes, Genes, Viral, Genetic Vectors, Molecular Sequence Data, Neutralization Tests, Poliovirus, Poliovirus Vaccine, Oral, Restriction Mapping",

author = "Burke, \{K L\} and Evans, \{D J\} and O Jenkins and J Meredith and D'Souza, \{E D\} and Almond, \{J W\}",

year = "1989",

month = sep,

language = "English",

volume = "70 ( Pt 9)",

pages = "2475--9",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Microbiology Society",

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TY - JOUR

T1 - A cassette vector for the construction of antigen chimaeras of poliovirus

AU - Burke, K L

AU - Evans, D J

AU - Jenkins, O

AU - Meredith, J

AU - D'Souza, E D

AU - Almond, J W

PY - 1989/9

Y1 - 1989/9

N2 - A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.

AB - A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.

KW - Amino Acid Sequence

KW - Antigens, Viral

KW - Base Sequence

KW - Chimera

KW - DNA, Viral

KW - Epitopes

KW - Genes, Viral

KW - Genetic Vectors

KW - Molecular Sequence Data

KW - Neutralization Tests

KW - Poliovirus

KW - Poliovirus Vaccine, Oral

KW - Restriction Mapping

UR - https://www.scopus.com/pages/publications/0024432624

M3 - Article

C2 - 2476528

SN - 0022-1317

VL - 70 ( Pt 9)

SP - 2475

EP - 2479

JO - Journal of General Virology

JF - Journal of General Virology

ER -

A cassette vector for the construction of antigen chimaeras of poliovirus

Abstract

UN SDGs

Keywords

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