A cassette vector for the construction of antigen chimaeras of poliovirus

K L Burke, D J Evans, O Jenkins, J Meredith, E D D'Souza, J W Almond

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.
Original languageEnglish
Pages (from-to)2475-9
Number of pages5
JournalJournal of General Virology
Volume70 ( Pt 9)
Publication statusPublished - Sept 1989

Keywords

  • Amino Acid Sequence
  • Antigens, Viral
  • Base Sequence
  • Chimera
  • DNA, Viral
  • Epitopes
  • Genes, Viral
  • Genetic Vectors
  • Molecular Sequence Data
  • Neutralization Tests
  • Poliovirus
  • Poliovirus Vaccine, Oral
  • Restriction Mapping

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