A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

John F. Atkins; Norma M. Wills; Gary Loughran; Chih-Yu Wu; Krishna Parsawar; Martin D. Ryan; Chung-Hsiung Wang; Chad C. Nelson

doi:10.1261/rna.487907

A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

John F. Atkins, Norma M. Wills, Gary Loughran, Chih-Yu Wu, Krishna Parsawar, Martin D. Ryan, Chung-Hsiung Wang, Chad C. Nelson

Research output: Contribution to journal › Article › peer-review

Abstract

When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.

Original language	English
Pages (from-to)	803-810
Number of pages	8
Journal	RNA
Volume	13
Early online date	24 Apr 2007
DOIs	https://doi.org/10.1261/rna.487907
Publication status	Published - Jun 2007

Keywords

codon redefinition
recoding
ribosome
picorna
2A
StopGo
PICORNA-LIKE-VIRUS
PEPTIDYL TRANSFERASE CENTER
TRANSFER-RNA
AMINO-ACID
NASCENT PEPTIDE
MESSENGER-RNA
A-SITE
CLEAVAGE ACTIVITIES
ECTROPIS-OBLIQUA
BOND FORMATION

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10.1261/rna.487907

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title = "A case for {"}StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)",

abstract = "When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.",

keywords = "codon redefinition, recoding, ribosome, picorna, 2A, StopGo, PICORNA-LIKE-VIRUS, PEPTIDYL TRANSFERASE CENTER, TRANSFER-RNA, AMINO-ACID, NASCENT PEPTIDE, MESSENGER-RNA, A-SITE, CLEAVAGE ACTIVITIES, ECTROPIS-OBLIQUA, BOND FORMATION",

author = "Atkins, {John F.} and Wills, {Norma M.} and Gary Loughran and Chih-Yu Wu and Krishna Parsawar and Ryan, {Martin D.} and Chung-Hsiung Wang and Nelson, {Chad C.}",

year = "2007",

month = jun,

doi = "10.1261/rna.487907",

language = "English",

volume = "13",

pages = "803--810",

journal = "RNA",

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publisher = "Cold Spring Harbor Laboratory Press",

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T1 - A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

AU - Atkins, John F.

AU - Wills, Norma M.

AU - Loughran, Gary

AU - Wu, Chih-Yu

AU - Parsawar, Krishna

AU - Ryan, Martin D.

AU - Wang, Chung-Hsiung

AU - Nelson, Chad C.

PY - 2007/6

Y1 - 2007/6

N2 - When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.

AB - When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.

KW - codon redefinition

KW - recoding

KW - ribosome

KW - picorna

KW - 2A

KW - StopGo

KW - PICORNA-LIKE-VIRUS

KW - PEPTIDYL TRANSFERASE CENTER

KW - TRANSFER-RNA

KW - AMINO-ACID

KW - NASCENT PEPTIDE

KW - MESSENGER-RNA

KW - A-SITE

KW - CLEAVAGE ACTIVITIES

KW - ECTROPIS-OBLIQUA

KW - BOND FORMATION

UR - http://www.scopus.com/inward/record.url?scp=34249092656&partnerID=8YFLogxK

U2 - 10.1261/rna.487907

DO - 10.1261/rna.487907

M3 - Article

SN - 1355-8382

VL - 13

SP - 803

EP - 810

JO - RNA

JF - RNA

ER -

A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

Abstract

Keywords

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