A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

John F. Atkins, Norma M. Wills, Gary Loughran, Chih-Yu Wu, Krishna Parsawar, Martin D. Ryan, Chung-Hsiung Wang, Chad C. Nelson

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.

Original languageEnglish
Pages (from-to)803-810
Number of pages8
JournalRNA
Volume13
Early online date24 Apr 2007
DOIs
Publication statusPublished - Jun 2007

Keywords

  • codon redefinition
  • recoding
  • ribosome
  • picorna
  • 2A
  • StopGo
  • PICORNA-LIKE-VIRUS
  • PEPTIDYL TRANSFERASE CENTER
  • TRANSFER-RNA
  • AMINO-ACID
  • NASCENT PEPTIDE
  • MESSENGER-RNA
  • A-SITE
  • CLEAVAGE ACTIVITIES
  • ECTROPIS-OBLIQUA
  • BOND FORMATION

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