5-hydroxymethylcytosine profiling as an indicator of cellular state

Alexander Laird; John P. Thomson; David J. Harrison; Richard R. Meehan

doi:10.2217/epi.13.69

5-hydroxymethylcytosine profiling as an indicator of cellular state

Alexander Laird
, John P. Thomson
, David J. Harrison
, Richard R. Meehan^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

DNA methylation is widely studied in the context of cancer. However, the rediscovery of 5-hydroxymethylation of DNA adds a new layer of complexity to understanding the epigenetic basis of development and disease, including carcinogenesis. There have been significant advances in techniques for the detection of 5-hydroxymethylcytosine and, with this, greater insight into the distribution, regulation and function of this mark, which are reviewed here. Better understanding of the associated pathways involved in regulation of, and by, 5-hydroxymethylcytosine may give promise to new therapeutic targets. We discuss evidence to support the view of 5-hydroxymethylcytosine as a unique and dynamic mark of cellular state. These 5-hydroxymethylcytosine profiles may offer optimism for the development of diagnostic, prognostic and predictive biomarkers.

Original language	English
Pages (from-to)	655-669
Number of pages	15
Journal	Epigenomics
Volume	5
Issue number	6
DOIs	https://doi.org/10.2217/epi.13.69
Publication status	Published - Dec 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Cellular state
Epigenetics
Hydroxymethylcytosine
Embryonic stem-cells
Active DNA demethylation
Acetylglucosamine transferase OGT
Isocitrate dehydrogenase 1
Acute myeloid-leukemia
Base excision-repair
Mouse-liver tumors
TET proteins
Thymine DNA
Human cancers

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laird2013epigenomics655
© Richard R Meehan et al. This work is licensed under the Creative Commons Attribution-NonCommercial 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0
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title = "5-hydroxymethylcytosine profiling as an indicator of cellular state",

abstract = "DNA methylation is widely studied in the context of cancer. However, the rediscovery of 5-hydroxymethylation of DNA adds a new layer of complexity to understanding the epigenetic basis of development and disease, including carcinogenesis. There have been significant advances in techniques for the detection of 5-hydroxymethylcytosine and, with this, greater insight into the distribution, regulation and function of this mark, which are reviewed here. Better understanding of the associated pathways involved in regulation of, and by, 5-hydroxymethylcytosine may give promise to new therapeutic targets. We discuss evidence to support the view of 5-hydroxymethylcytosine as a unique and dynamic mark of cellular state. These 5-hydroxymethylcytosine profiles may offer optimism for the development of diagnostic, prognostic and predictive biomarkers.",

keywords = "Cancer, Cellular state, Epigenetics, Hydroxymethylcytosine, Embryonic stem-cells, Active DNA demethylation, Acetylglucosamine transferase OGT, Isocitrate dehydrogenase 1, Acute myeloid-leukemia, Base excision-repair, Mouse-liver tumors, TET proteins, Thymine DNA, Human cancers",

author = "Alexander Laird and Thomson, \{John P.\} and Harrison, \{David J.\} and Meehan, \{Richard R.\}",

note = "A Laird is supported by the Medical Research Council Scottish Clinical Pharmacology and Pathology Programme, The Royal College of Surgeons of Edinburgh Robertson{\textquoteright}s Trust and The Melville Trust for the Care and Cure of Cancer. J Thomson is supported by the MARCAR project. Work in RR Meehan{\textquoteright}s laboratory is supported by the Medical Research Council, the BBSRC and by the Innovative Medicine Initiative Joint Undertaking (IMI JU) under grant agreement number 115001 (MARCAR project).",

year = "2013",

month = dec,

doi = "10.2217/epi.13.69",

language = "English",

volume = "5",

pages = "655--669",

journal = "Epigenomics",

issn = "1750-1911",

publisher = "Taylor and Francis",

number = "6",

}

TY - JOUR

T1 - 5-hydroxymethylcytosine profiling as an indicator of cellular state

AU - Laird, Alexander

AU - Thomson, John P.

AU - Harrison, David J.

AU - Meehan, Richard R.

N1 - A Laird is supported by the Medical Research Council Scottish Clinical Pharmacology and Pathology Programme, The Royal College of Surgeons of Edinburgh Robertson’s Trust and The Melville Trust for the Care and Cure of Cancer. J Thomson is supported by the MARCAR project. Work in RR Meehan’s laboratory is supported by the Medical Research Council, the BBSRC and by the Innovative Medicine Initiative Joint Undertaking (IMI JU) under grant agreement number 115001 (MARCAR project).

PY - 2013/12

Y1 - 2013/12

N2 - DNA methylation is widely studied in the context of cancer. However, the rediscovery of 5-hydroxymethylation of DNA adds a new layer of complexity to understanding the epigenetic basis of development and disease, including carcinogenesis. There have been significant advances in techniques for the detection of 5-hydroxymethylcytosine and, with this, greater insight into the distribution, regulation and function of this mark, which are reviewed here. Better understanding of the associated pathways involved in regulation of, and by, 5-hydroxymethylcytosine may give promise to new therapeutic targets. We discuss evidence to support the view of 5-hydroxymethylcytosine as a unique and dynamic mark of cellular state. These 5-hydroxymethylcytosine profiles may offer optimism for the development of diagnostic, prognostic and predictive biomarkers.

AB - DNA methylation is widely studied in the context of cancer. However, the rediscovery of 5-hydroxymethylation of DNA adds a new layer of complexity to understanding the epigenetic basis of development and disease, including carcinogenesis. There have been significant advances in techniques for the detection of 5-hydroxymethylcytosine and, with this, greater insight into the distribution, regulation and function of this mark, which are reviewed here. Better understanding of the associated pathways involved in regulation of, and by, 5-hydroxymethylcytosine may give promise to new therapeutic targets. We discuss evidence to support the view of 5-hydroxymethylcytosine as a unique and dynamic mark of cellular state. These 5-hydroxymethylcytosine profiles may offer optimism for the development of diagnostic, prognostic and predictive biomarkers.

KW - Cancer

KW - Cellular state

KW - Epigenetics

KW - Hydroxymethylcytosine

KW - Embryonic stem-cells

KW - Active DNA demethylation

KW - Acetylglucosamine transferase OGT

KW - Isocitrate dehydrogenase 1

KW - Acute myeloid-leukemia

KW - Base excision-repair

KW - Mouse-liver tumors

KW - TET proteins

KW - Thymine DNA

KW - Human cancers

U2 - 10.2217/epi.13.69

DO - 10.2217/epi.13.69

M3 - Article

SN - 1750-1911

VL - 5

SP - 655

EP - 669

JO - Epigenomics

JF - Epigenomics

IS - 6

ER -

5-hydroxymethylcytosine profiling as an indicator of cellular state

Abstract

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Keywords

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