2A to the Fore - Research, Technology and Applications

Garry A. Luke; Helena Escuin; Pablo De Felipe; Martin D. Ryan

doi:10.5661/bger-26-223

2A to the Fore - Research, Technology and Applications

Garry A. Luke, Helena Escuin, Pablo De Felipe, Martin D. Ryan

Research output: Chapter in Book/Report/Conference proceeding › Other contribution

Abstract

The 2A region of the foot-and-mouth disease virus (FMDV) encodes a short sequence that mediates self-processing by a novel translational effect. Translation elongation arrest leads to release of the nascent polypeptide and re-initiation at the next in-frame codon. In this way discrete translation products are derived from a single open reading frame. Active 2A-like sequences have been found in (many) other viruses and trypanosome non-LTR retrotransposons. Exponential growth of 2A technology within the last decade has lead to many biotechnological/biomedical applications including the generation of transgenic plants/animals and genetic manipulation of human embryonic stem cells (hESCs).

Original language	English
Title of host publication	Biotechnology and Genetic Engineering Reviews
Editors	Stephen Harding
Place of Publication	Loughborough
Publisher	Nottingham University Press
Pages	223-260
Number of pages	38
Volume	26
DOIs	https://doi.org/10.5661/bger-26-223
Publication status	Published - 1 Nov 2009

Keywords

MOUTH-DISEASE VIRUS
PICORNA-LIKE-VIRUS
COMPLETE NUCLEOTIDE-SEQUENCE
PLURIPOTENT STEM-CELLS
GROUP-C ROTAVIRUS
SIGNAL RECOGNITION PARTICLE
ENZYME-REPLACEMENT THERAPY
GREEN FLUORESCENT PROTEIN
DEPENDENT RNA-POLYMERASES
RECEPTOR RETROGENIC MICE

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5661/bger-26-223

Cite this

@inbook{d13680791d7e43b4a36a705a2b11d3dd,

title = "2A to the Fore - Research, Technology and Applications",

abstract = "The 2A region of the foot-and-mouth disease virus (FMDV) encodes a short sequence that mediates self-processing by a novel translational effect. Translation elongation arrest leads to release of the nascent polypeptide and re-initiation at the next in-frame codon. In this way discrete translation products are derived from a single open reading frame. Active 2A-like sequences have been found in (many) other viruses and trypanosome non-LTR retrotransposons. Exponential growth of 2A technology within the last decade has lead to many biotechnological/biomedical applications including the generation of transgenic plants/animals and genetic manipulation of human embryonic stem cells (hESCs).",

keywords = "MOUTH-DISEASE VIRUS, PICORNA-LIKE-VIRUS, COMPLETE NUCLEOTIDE-SEQUENCE, PLURIPOTENT STEM-CELLS, GROUP-C ROTAVIRUS, SIGNAL RECOGNITION PARTICLE, ENZYME-REPLACEMENT THERAPY, GREEN FLUORESCENT PROTEIN, DEPENDENT RNA-POLYMERASES, RECEPTOR RETROGENIC MICE",

author = "Luke, {Garry A.} and Helena Escuin and {De Felipe}, Pablo and Ryan, {Martin D.}",

year = "2009",

month = nov,

day = "1",

doi = "10.5661/bger-26-223",

language = "English",

volume = "26",

pages = "223--260",

editor = "Stephen Harding",

booktitle = "Biotechnology and Genetic Engineering Reviews",

publisher = "Nottingham University Press",

address = "United Kingdom",

}

TY - CHAP

T1 - 2A to the Fore - Research, Technology and Applications

AU - Luke, Garry A.

AU - Escuin, Helena

AU - De Felipe, Pablo

AU - Ryan, Martin D.

PY - 2009/11/1

Y1 - 2009/11/1

N2 - The 2A region of the foot-and-mouth disease virus (FMDV) encodes a short sequence that mediates self-processing by a novel translational effect. Translation elongation arrest leads to release of the nascent polypeptide and re-initiation at the next in-frame codon. In this way discrete translation products are derived from a single open reading frame. Active 2A-like sequences have been found in (many) other viruses and trypanosome non-LTR retrotransposons. Exponential growth of 2A technology within the last decade has lead to many biotechnological/biomedical applications including the generation of transgenic plants/animals and genetic manipulation of human embryonic stem cells (hESCs).

AB - The 2A region of the foot-and-mouth disease virus (FMDV) encodes a short sequence that mediates self-processing by a novel translational effect. Translation elongation arrest leads to release of the nascent polypeptide and re-initiation at the next in-frame codon. In this way discrete translation products are derived from a single open reading frame. Active 2A-like sequences have been found in (many) other viruses and trypanosome non-LTR retrotransposons. Exponential growth of 2A technology within the last decade has lead to many biotechnological/biomedical applications including the generation of transgenic plants/animals and genetic manipulation of human embryonic stem cells (hESCs).

KW - MOUTH-DISEASE VIRUS

KW - PICORNA-LIKE-VIRUS

KW - COMPLETE NUCLEOTIDE-SEQUENCE

KW - PLURIPOTENT STEM-CELLS

KW - GROUP-C ROTAVIRUS

KW - SIGNAL RECOGNITION PARTICLE

KW - ENZYME-REPLACEMENT THERAPY

KW - GREEN FLUORESCENT PROTEIN

KW - DEPENDENT RNA-POLYMERASES

KW - RECEPTOR RETROGENIC MICE

U2 - 10.5661/bger-26-223

DO - 10.5661/bger-26-223

M3 - Other contribution

VL - 26

SP - 223

EP - 260

BT - Biotechnology and Genetic Engineering Reviews

A2 - Harding, Stephen

PB - Nottingham University Press

CY - Loughborough

ER -

2A to the Fore - Research, Technology and Applications

Abstract

Keywords

UN SDGs

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