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Abstract
We report the initial characterisation of an N-terminal oligopeptide ‘2A-like’ sequence that is able to function both as a signal sequence and as a translational recoding element. Due to this translational recoding activity, two forms of nascent polypeptide are synthesised: (i) when 2A-mediated translational recoding has not occurred: the nascent polypeptide is fused to the 2A-like N-terminal signal sequence and the fusion translation product is targeted to the exocytic pathway, and, (ii) a translation product where 2A-mediated translational recoding has occurred: the 2A-like signal sequence is synthesised as a separate translation product and, therefore, the nascent (downstream) polypeptide lacks the 2A-like signal sequence and is localised to the cytoplasm. This type of dual-functional signal sequence results, therefore, in the partitioning of the translation products between the two sub-cellular sites and represents a newly described form of dual protein targeting.
Original language | English |
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Pages (from-to) | 923-939 |
Number of pages | 17 |
Journal | Traffic |
Volume | 17 |
Issue number | 8 |
Early online date | 2 Jun 2016 |
DOIs | |
Publication status | Published - 13 Jul 2016 |
Keywords
- Translational recoding
- 2A
- Signal sequence
- Secretory pathway
- Dual protein targeting
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Dive into the research topics of '"2A-like" signal sequences mediating translational recoding: a novel form of dual protein targeting'. Together they form a unique fingerprint.Projects
- 2 Finished
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Processes that shape continental record: The Processes that shape the Continental Record
Cawood, P. A. (PI), Cawood, P. A. (PI) & Hawkesworth, C. (CoI)
1/07/12 → 31/12/15
Project: Standard
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Molecular Charactorisation of Leader: Molecular characterisation of leader sequences which can also mediate ribosome skipping
Ryan, M. D. (PI)
1/04/10 → 31/05/13
Project: Standard