2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

Marius M. Haugland; Afaf H. El-Sagheer; Rachel J. Porter; Javier Peña; Tom Brown; Edward A. Anderson; Janet E. Lovett

doi:10.1021/jacs.6b05421

2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

Marius M. Haugland, Afaf H. El-Sagheer, Rachel J. Porter, Javier Peña, Tom Brown^*, Edward A. Anderson, Janet E. Lovett

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

5 Downloads (Pure)

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.

Original language	English
Pages (from-to)	9069-9072
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	138
Issue number	29
Early online date	13 Jul 2016
DOIs	https://doi.org/10.1021/jacs.6b05421
Publication status	Published - 27 Jul 2016

Access to Document

10.1021/jacs.6b05421Licence: CC BY

Haughland_2016_EPR_JACS_9069
Copyright © 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 1.36 MBLicence: CC BY

New Strategies for Spin Labelling: New strategies for spin labelling cysteine-rich proteins
Lovett, J. E. (PI)
EPSRC
1/09/14 → 31/08/15
Project: Standard
Royal Society Research Fellowship: Royal Society Research Fellowship
Lovett, J. E. (PI)
The Royal Society
1/05/14 → 15/02/17
Project: Fellowship
State of the art pulse EPR instrumentati: State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules
Smith, G. M. (PI), Bode, B. E. (CoI), Naismith, J. (CoI), Schiemann, O. (CoI) & White, M. (CoI)
The Wellcome Trust
1/09/12 → 31/08/17
Project: Standard

Janet Eleanor Lovett
- jel20st-andrews.acuk
- School of Physics and Astronomy - Senior Lecturer
- Biomedical Sciences Research Complex
Person: Academic, Academic - Research

Data underpinning - 2′-Alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA
Haugland, M. (Creator), El-Sagheer, A. (Creator), Porter, R. (Creator), Pena, J. (Creator), Brown, T. (Creator), Anderson, E. (Creator) & Lovett, J. E. (Creator), University of St Andrews, 16 Aug 2016
DOI: 10.17630/764e7545-41b4-4a92-ab23-552a22050fe9
Dataset

File

Cite this

@article{1378c09e52ea41468ece5d4be0c4a2cb,

title = "2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA",

abstract = "Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.",

author = "Haugland, {Marius M.} and El-Sagheer, {Afaf H.} and Porter, {Rachel J.} and Javier Pe{\~n}a and Tom Brown and Anderson, {Edward A.} and Lovett, {Janet E.}",

note = "TB and AHE-S thank the BBSRC (sLOLA grant BB/J001694/1). EAA thanks the EPSRC (EP/K005391/1, EP/M019195/1). JEL thanks the EPSRC (EP/LO22044/1), and the Royal Society for a University Research Fellowship and grant RF120645. We thank the Wellcome Trust for the Q-band EPR spectrometer (099149/Z/12/Z), the EPSRC National EPR Service for spectrometer time, and Dr Hassane El Mkami for technical assistance.",

year = "2016",

month = jul,

day = "27",

doi = "10.1021/jacs.6b05421",

language = "English",

volume = "138",

pages = "9069--9072",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society (ACS)",

number = "29",

}

TY - JOUR

T1 - 2′-alkynylnucleotides

T2 - a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

AU - Haugland, Marius M.

AU - El-Sagheer, Afaf H.

AU - Porter, Rachel J.

AU - Peña, Javier

AU - Brown, Tom

AU - Anderson, Edward A.

AU - Lovett, Janet E.

N1 - TB and AHE-S thank the BBSRC (sLOLA grant BB/J001694/1). EAA thanks the EPSRC (EP/K005391/1, EP/M019195/1). JEL thanks the EPSRC (EP/LO22044/1), and the Royal Society for a University Research Fellowship and grant RF120645. We thank the Wellcome Trust for the Q-band EPR spectrometer (099149/Z/12/Z), the EPSRC National EPR Service for spectrometer time, and Dr Hassane El Mkami for technical assistance.

PY - 2016/7/27

Y1 - 2016/7/27

N2 - Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.

AB - Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.

U2 - 10.1021/jacs.6b05421

DO - 10.1021/jacs.6b05421

M3 - Article

AN - SCOPUS:84979955517

SN - 0002-7863

VL - 138

SP - 9069

EP - 9072

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 29

ER -

2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

Abstract

Access to Document

Fingerprint

Projects

New Strategies for Spin Labelling: New strategies for spin labelling cysteine-rich proteins

Royal Society Research Fellowship: Royal Society Research Fellowship

State of the art pulse EPR instrumentati: State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules

Profiles

Janet Eleanor Lovett

Datasets

Data underpinning - 2′-Alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA

Cite this