2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

Marius M. Haugland, Afaf H. El-Sagheer, Rachel J. Porter, Javier Peña, Tom Brown*, Edward A. Anderson, Janet E. Lovett

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.

Original languageEnglish
Pages (from-to)9069-9072
Number of pages4
JournalJournal of the American Chemical Society
Volume138
Issue number29
Early online date13 Jul 2016
DOIs
Publication statusPublished - 27 Jul 2016

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