17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

Sonja Zafirovic; Milan Obradovic; Emina Sudar-Milovanovic; Aleksandra Jovanovic; Julijana Stanimirovic; Alan J. Stewart; Samantha J. Pitt; Esma R. Isenovic

doi:10.1016/j.mce.2017.02.001

17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

Sonja Zafirovic, Milan Obradovic, Emina Sudar-Milovanovic, Aleksandra Jovanovic, Julijana Stanimirovic, Alan J. Stewart, Samantha J. Pitt, Esma R. Isenovic

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to investigate the in vivo effects of estradiol (E₂) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E₂ while the other half were placebo-treated. 24h after treatment animals were sacrificed. E₂ reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr³⁰⁸ (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E₂ tretment in HF rats. Our results indicate that E₂ acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.

Original language	English
Pages (from-to)	12-20
Number of pages	9
Journal	Molecular and Cellular Endocrinology
Volume	446
Early online date	2 Feb 2017
DOIs	https://doi.org/10.1016/j.mce.2017.02.001
Publication status	Published - 5 May 2017

Keywords

Estradiol
Obesity
Myocardial metabolism
Cardiovascular diseases
Inducible nitric oxide synthase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mce.2017.02.001

Stewart_2017_MCE_HighFatDiet_AAM
Copyright © 2017, Elsevier Ltd. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.mce.2017.02.001
Accepted author manuscript, 1.39 MBLicence: CC BY-NC-ND

Cite this

@article{39cf3c6ce256400baf562299711c774f,

title = "17{\ss}-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats",

abstract = "The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.",

keywords = "Estradiol, Obesity, Myocardial metabolism, Cardiovascular diseases, Inducible nitric oxide synthase",

author = "Sonja Zafirovic and Milan Obradovic and Emina Sudar-Milovanovic and Aleksandra Jovanovic and Julijana Stanimirovic and Stewart, {Alan J.} and Pitt, {Samantha J.} and Isenovic, {Esma R.}",

note = "This work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia.",

year = "2017",

month = may,

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doi = "10.1016/j.mce.2017.02.001",

language = "English",

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journal = "Molecular and Cellular Endocrinology",

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TY - JOUR

T1 - 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

AU - Zafirovic, Sonja

AU - Obradovic, Milan

AU - Sudar-Milovanovic, Emina

AU - Jovanovic, Aleksandra

AU - Stanimirovic, Julijana

AU - Stewart, Alan J.

AU - Pitt, Samantha J.

AU - Isenovic, Esma R.

N1 - This work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia.

PY - 2017/5/5

Y1 - 2017/5/5

N2 - The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.

AB - The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.

KW - Estradiol

KW - Obesity

KW - Myocardial metabolism

KW - Cardiovascular diseases

KW - Inducible nitric oxide synthase

UR - http://www.sciencedirect.com/science/article/pii/S0303720717300771

U2 - 10.1016/j.mce.2017.02.001

DO - 10.1016/j.mce.2017.02.001

M3 - Article

SN - 0303-7207

VL - 446

SP - 12

EP - 20

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

ER -

17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

Abstract

Keywords

UN SDGs

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