State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules

2025

Robustness and sensitivity of Gd(III)-Gd(III) double electron electron resonance (DEER) measurements: comparative study of high-frequency EPR spectrometer designs and spin label variants

Mocanu, E. M., Ben-Ishay, Y., Topping, L., Fisher, S. R., Hunter, R. I., Su, X.-C., Butler, S., Smith, G. M., Goldfarb, D. & Lovett, J. E., 3 Jan 2025, (E-pub ahead of print) In: Applied Magnetic Resonance. Early View, 21 p.

Research output: Contribution to journal › Article › peer-review

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2024

Pulse dipolar electron paramagnetic resonance spectroscopy distance measurements at low nanomolar concentrations: the Cu^II-trityl case

Ackermann, K., Heubach, C., Schiemann, O. & Bode, B. E., 31 Jan 2024, (E-pub ahead of print) In: The Journal of Physical Chemistry Letters. 15, p. 1455-1461 7 p.

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Spirocyclic pyrrolidinyl nitroxides with exo-methylene substituents

Sowiński, M. P., Warnke, A.-L., Lund, B. A., Skagseth, S., Cordes, D. B., Lovett, J. E. & Haugland, M. M., 13 Dec 2024, In: ChemPlusChem. 89, 12, 7 p., e202400387.

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2023

Conformational tuning improves the stability of spirocyclic nitroxides with long paramagnetic relaxation times

Sowiński, M. P., Gahlawat, S., Lund, B. A., Warnke, A.-L., Hopmann, K. H., Lovett, J. E. & Haugland, M. M., 5 Jun 2023, In: Communications Chemistry. 6, 1, 8 p., 111.

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Darobactin B stabilises a lateral-closed conformation of the BAM complex in E. coli cells

Haysom, S. F., Machin, J., Whitehouse, J. M., Horne, J. E., Fenn, K., Ma, Y., El Mkami, H., Böhringer, N., Schäberle, T. F., Ranson, N. A., Radford, S. E. & Pliotas, C., 21 Aug 2023, In: Angewandte Chemie International Edition. 62, 34, 8 p., e202218783.

Research output: Contribution to journal › Article › peer-review

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Design of the elusive proteinaceous oxygen donor copper site suggests a promising future for copper for MRI contrast agents

Shah, A., Taylor, M. J., Molinaro, G., Anbu, S., Verdu, M., Jennings, L., Mikulska, I., Diaz-Moreno, S., El Mkami, H., Smith, G. M., Britton, M. M., Lovett, J. E. & Peacock, A. F. A., 4 Jul 2023, In: Proceedings of the National Academy of Sciences of the United States of America. 120, 27, e2219036120.

Research output: Contribution to journal › Article › peer-review

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Large cross-effect dynamic nuclear polarisation enhancements with kilowatt inverting chirped pulses at 94 GHz

Zhao, Y., El Mkami, H., Hunter, R. I., Casano, G., Ouari, O. & Smith, G. M., 22 Aug 2023, In: Communications Chemistry. 6, 12 p., 171.

Research output: Contribution to journal › Article › peer-review

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2022

Combined pulsed electron double resonance EPR and molecular dynamics investigations of calmodulin suggest effects of crowding agents on protein structures

Stewart, A., Shanmugam, M., Kutta, R., Scrutton, N., Lovett, J. E. & Hay, S., 6 Sept 2022, In: Biochemistry. 61, 17, p. 1735–1742 8 p.

Research output: Contribution to journal › Article › peer-review

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2021

A comparison of cysteine-conjugated nitroxide spin labels for pulse dipolar EPR spectroscopy

Ackermann, K., Chapman, A. & Bode, B. E., 13 Dec 2021, In: Molecules. 26, 24, 16 p., 7534.

Research output: Contribution to journal › Article › peer-review

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A general model to optimise Cu^II labelling efficiency of double-histidine motifs for pulse dipolar EPR applications

Wort, J., Ackermann, K., Norman, D. & Bode, B. E., 14 Feb 2021, In: Physical Chemistry Chemical Physics. 23, 6, p. 3810-3819 10 p.

Research output: Contribution to journal › Article › peer-review

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DEER and RIDME measurements of the nitroxide-spin labelled copper-bound amine oxidase homodimer from arthrobacter globiformis

Russell, H., Stewart, R., Prior, C., Oganesyan, V. S., Gaule, T. G. & Lovett, J. E., 29 Mar 2021, (E-pub ahead of print) In: Applied Magnetic Resonance. First Online, 21 p.

Research output: Contribution to journal › Article › peer-review

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Nanomolar pulse dipolar EPR spectroscopy in proteins: Cu^II-Cu^II and nitroxide-nitroxide cases

Ackermann, K., Wort, J. & Bode, B. E., 2021, In: Journal of Physical Chemistry B. Articles ASAP

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Pulse dipolar EPR reveals double-histidine motif Cu^II-NTA spin-labelling robustness against competitor ions

Wort, J., Arya, S., Ackermann, K., Stewart, A. J. & Bode, B. E., 25 Mar 2021, In: Journal of Physical Chemistry Letters. 12, 11, p. 2815-2819 5 p.

Research output: Contribution to journal › Article › peer-review

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2020

2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis

Hardwick, J., Haugland, M., El-Sagheer, A., Ptchelkine, D., Beierlein, F., Lane, A., Brown, T., Lovett, J. E. & Anderson, E., 13 Feb 2020, (E-pub ahead of print) In: Nucleic Acids Research. Advance articles, gkaa086.

Research output: Contribution to journal › Article › peer-review

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Advanced EPR spectroscopy for investigation of biomolecular binding events

Wort, J., Oranges, M., Ackermann, K. & Bode, B. E., 2020, Electron Paramagnetic Resonance: Volume 27. Murphy, D., Chechik, V. & Bode, B. (eds.). Royal Society of Chemistry (RSC), Vol. 27. p. 47-73 27 p. (SPR - Electron Paramagnetic Resonance).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

High-sensitivity Gd³⁺-Gd³⁺ EPR distance measurements that eliminate artefacts seen at short distances

El Mkami, H., Hunter, R. I., Cruickshank, P. A. S., Taylor, M. J., Lovett, J. E., Feintuch, A., Qi, M., Godt, A. & Smith, G. M., 9 Dec 2020, In: Magnetic Resonance. 1, 2, p. 301-313

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In-lipid structure of pressure sensitive domains hints mechanosensitive channel functional diversity

Kapsalis, C., Ma, Y., Bode, B. E. & Pliotas, C., 21 Jul 2020, In: Biophysical Journal. 119, 2, p. 448-459

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Iron is a ligand of SecA-like metal-binding domains in vivo

Cranford-Smith, T., Jamshad, M., Jeeves, M., Chandler, R. A., Yule, J., Robinson, A., Alam, F., Dunne, K. A., Aponte Angarita, E. H., Alanazi, M., Carter, C., Henderson, I. R., Lovett, J. E., Winn, P., Knowles, T. & Huber, D., 2 Apr 2020, (E-pub ahead of print) In: Journal of Biological Chemistry. Papers in Press

Research output: Contribution to journal › Article › peer-review

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2019

A gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurements

Shah, A., Roux, A., Starck, M., Mosely, J. A., Stevens, M., Norman, D. G., Hunter, R. I., El Mkami, H., Smith, G., Parker, D. & Lovett, J. E., 18 Feb 2019, (E-pub ahead of print) In: Inorganic Chemistry. Article ASAP

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Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets

Kapsalis, C., Wang, B., El Mkami, H., Pitt, S. J., Schnell, J., Smith, T. K., Lippiat, J., Bode, B. E. & Pliotas, C., 10 Oct 2019, In: Nature Communications. 10, 14 p., 4619.

Research output: Contribution to journal › Article › peer-review

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ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery

Corey, R., Ahdash, Z., Shah, A., Pyle, E., Allen, W., Fessl, T., Lovett, J. E., Politis, A. & Collinson, I., 2 Jan 2019, In: eLife. 8, 25 p., e41803.

Research output: Contribution to journal › Article › peer-review

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Sub-micromolar pulse dipolar EPR spectroscopy reveals increasing Cu^II-labelling of double-histidine motifs with lower temperature

Wort, J. L., Ackermann, K., Giannoulis, A., Stewart, A. J., Norman, D. G. & Bode, B. E., 19 Aug 2019, In: Angewandte Chemie - International Edition. 58, 34, p. 11681-11685 5 p.

Research output: Contribution to journal › Article › peer-review

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Sub-micromolar pulse dipolar EPR spectroscopy reveals increasing Cu^II-labelling of double-histidine motifs with lower temperature

Wort, J., Ackermann, K., Giannoulis, A., Stewart, A. J., Norman, D. & Bode, B. E., 19 Aug 2019, In: Angewandte Chemie. 131, 34, p. 11807-11811 6 p.

Research output: Contribution to journal › Article › peer-review

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2018

Distance measurement of a noncovalently bound Y@C₈₂ Pair with double electron electron resonance spectroscopy

Gil-Ramírez, G., Shah, A., El Mkami, H., Porfyrakis, K., Briggs, A. D., Morton, J. J. L., Anderson, H. L. & Lovett, J. E., 20 Jun 2018, In: Journal of the American Chemical Society. 140, 24, p. 7420-7424

Research output: Contribution to journal › Article › peer-review

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Nitroxide-nitroxide and nitroxide-metal distance measurements in transition metal complexes with two or three paramagnetic centres give access to thermodynamic and kinetic stabilities

Giannoulis, A., Ackermann, K., Spindler, P., Higgins, C., Cordes, D. B., Slawin, A. M. Z., Prisner, T. F. & Bode, B. E., 28 Apr 2018, In: Physical Chemistry Chemical Physics. 20, 16, p. 11196-11205 10 p.

Research output: Contribution to journal › Article › peer-review

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Orientation selection in high-field RIDME and PELDOR experiments involving low-spin Co^II ions

Giannoulis, A., Motion, C. L., Oranges, M., Buehl, M., Smith, G. M. & Bode, B. E., 28 Jan 2018, In: Physical Chemistry Chemical Physics. 20, 4, p. 2151-2154

Research output: Contribution to journal › Article › peer-review

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Pulse EPR distance measurements to study multimers and multimerisation

Ackermann, K. & Bode, B. E., 2018, In: Molecular Physics. 116, 12, p. 1513-1521 9 p.

Research output: Contribution to journal › Article › peer-review

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Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome

Sundaramoorthy, R., Hughes, A. L., El-Mkami, H., Norman, D. G., Ferreira, H. & Owen-Hughes, T., 6 Aug 2018, In: eLife. 7, 28 p., e35720.

Research output: Contribution to journal › Article › peer-review

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2017

Air exposure induced recombination in PTB7:PC₇₁BM solar cells

Thomson, S. A. J., Hogg, S. C., Samuel, I. D. W. & Keeble, D. J., 7 Nov 2017, In: Journal of Materials Chemistry A. 5, 41, p. 21926-21935

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Cryo-EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation

Ilangovan, A., Kay, C. W. M., Roier, S., El Mkami, H., Salvadori, E., Zechner, E. L., Zanetti, G. & Waksman, G., 4 May 2017, In: Cell. 169, 4, p. 708-721 27 p., e12.

Research output: Contribution to journal › Article › peer-review

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Monitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurements

Giannoulis, A., Oranges, M. & Bode, B. E., 6 Sept 2017, In: ChemPhysChem. 18, 17, p. 2318-2321 5 p.

Research output: Contribution to journal › Article › peer-review

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Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels

Ackermann, K., Pliotas, C., Valera, S., Naismith, J. & Bode, B. E., 7 Nov 2017, In: Biophysical Journal. 113, 9, p. 1968–1978

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Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes

Sundaramoorthy, R., Hughes, A. L., Singh, V., Wiechens, N., Ryan, D. P., El-Mkami, H., Petoukhov, M., Svergun, D. I., Treutlein, B., Quack, S., Fischer, M., Michaelis, J., Böttcher, B., Norman, D. G. & Owen-Hughes, T., 23 Mar 2017, In: eLife. 6, 28 p., e22510.

Research output: Contribution to journal › Article › peer-review

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The use of composite pulses for improving DEER signal at 94 GHz

Motion, C. L., Cassidy, S. L., Cruickshank, P. A. S., Hunter, R. I., Bolton, D. R., El Mkami, H., Van Doorslaer, S., Lovett, J. E. & Smith, G. M., May 2017, In: Journal of Magnetic Resonance. 278, p. 122-133 12 p.

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2016

2′-alkynylnucleotides: a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

Haugland, M. M., El-Sagheer, A. H., Porter, R. J., Peña, J., Brown, T., Anderson, E. A. & Lovett, J. E., 27 Jul 2016, In: Journal of the American Chemical Society. 138, 29, p. 9069-9072 4 p.

Research output: Contribution to journal › Article › peer-review

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Accurate extraction of nanometer distances in multimers by pulse EPR

Valera, S., Ackermann, K., Pliotas, C., Huang, H., Naismith, J. H. & Bode, B. E., 16 Mar 2016, In: Chemistry - A European Journal. 22, 14, p. 4700–4703

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Analysis of the intrinsically disordered N-terminus of the DNA junction-resolving enzyme T7 endonuclease I: identification of structure formed upon DNA binding

Freeman, A. D. J., Stevens, M., Declais, A. C., Leahy, A., Mackay, K., El Mkami, H., Lilley, D. M. J. & Norman, D. G., 2 Aug 2016, In: Biochemistry. 55, 30, p. 4166-4172 7 p.

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DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR

Motion, C. L., Lovett, J. E., Bell, S., Cassidy, S. L., Cruickshank, P. A. S., Bolton, D. R., Hunter, R. I., El Mkami, H., Van Doorslaer, S. & Smith, G. M., 21 Apr 2016, In: Journal of Physical Chemistry Letters. 7, 8, p. 1411-1415 5 p.

Research output: Contribution to journal › Article › peer-review

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The histone chaperone Vps75 forms multiple oligomeric assemblies capable of mediating exchange between histone H3-H4 tetramers and Asf1-H3-H4 complexes

Hammond, C. M., Sundaramoorthy, R., Larance, M., Lamond, A., Stevens, M. A., El-Mkami, H., Norman, D. G. & Owen-Hughes, T., 27 Jul 2016, In: Nucleic Acids Research. 44, 13, p. 6157-6172 16 p.

Research output: Contribution to journal › Article › peer-review

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The use of the Rx spin label in orientation measurement on proteins, by EPR

Stevens, M. A., McKay, J. E., Robinson, J. L. S., El Mkami, H., Smith, G. M. & Norman, D. G., 28 Feb 2016, In: Physical Chemistry Chemical Physics. 18, 8, p. 5799-5806

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2015

Binding dynamics of a monomeric SSB protein to DNA: a single-molecule multi-process approach

Morten, M. J., Peregrina, J. R., Figueira-Gonzalez, M., Ackermann, K., Bode, B. E., White, M. F. & Penedo, C., 15 Dec 2015, In: Nucleic Acids Research. 43, 22, p. 10907-10924 18 p.

Research output: Contribution to journal › Article › peer-review

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2014

Analysis of influenza A virus NS1 dimer interfaces in solution by pulse EPR distance measurements

Kerry, P. S., Turkington, H. L., Ackermann, K., Jameison, S. A. & Bode, B. E., 22 Aug 2014, In: Journal of Physical Chemistry B. 118, 37, p. 10882-10888 7 p.

Research output: Contribution to journal › Article › peer-review

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State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules

Research output

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