Research output per year
Research output per year
Personal profile
Research overview
Research in the MacNeill lab is primarily focused on dissecting the enzymes and mechanisms of DNA replication and genome stability using two contrasting genetically-tractable model systems, the eukaryotic fission yeast Schizosaccharomyces pombe and the halophilic euryarchaeon Haloferax volcanii. In addition to this, we also study the molecular biology of T5-like bacteriophages, the activity of the highly diverged DNA ligase enzymes encoded by crAss-like bacteriophages and various eukaryotic viruses, such as Cedratvirus kamchatka, and the function of selected carbohydrate-active enzymes (CAZymes) in Haloarcula hispanica, Haloferax volcanii and related haloarchaea. In the past we have also worked on the parasitic protozoan Trypanosoma brucei and the non-human infectious kinetoplastid model organism Crithidia fasciculata. We use a variety of methods to address questions of protein structure and function, including genetics, genome engineering, cell and molecular biology, biochemistry, structural biology and bioinformatics.
Academic/Professional Qualification
B.Sc. Honours (1st class) in Molecular Biology, University of Glasgow 1986
D.Phil. in Biochemistry, University of Oxford 1990
Teaching activity
BL2300 Research methods in biology
BL2301 Cell biology
BL2302 Molecular biology
BL3302 Gene regulation
BL4223 Molecular and cell biology of eukaryotic DNA replication
BL4201 Experimental research project
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Fingerprint
Dive into the research topics where Stuart MacNeill is active. These topic labels come from the works of this person. Together they form a unique fingerprint.
- 1 Similar Profiles
-
The archaeal RecJ-like proteins: nucleases and ex-nucleases with diverse roles in replication and repair
MacNeill, S. A., Dec 2018, In: Emerging Topics in Life Sciences. 2, 4, p. 493-501Research output: Contribution to journal › Review article › peer-review
Open AccessFile5 Citations (Scopus)6 Downloads (Pure) -
A novel archaeal DNA repair factor that acts with the UvrABC system to repair mitomycin C-induced DNA damage in a PCNA-dependent manner
Giroux, X. & MacNeill, S., 22 Dec 2015, In: Molecular Microbiology. 99, 1, p. 1-14Research output: Contribution to journal › Article › peer-review
Open AccessFile7 Citations (Scopus)5 Downloads (Pure) -
Canonical binding of Chaetomium thermophilum DNA polymerase δ/ζ subunit PolD3 and flap endonuclease Fen1 to PCNA
Alphey, M. S., Wolford, C. & MacNeill, S., 18 Dec 2023, In: Frontiers in Molecular Biosciences. 10, 9 p., 1320648.Research output: Contribution to journal › Article › peer-review
Open AccessFile4 Downloads (Pure) -
Remote homology detection identifies a eukaryotic RPA DBD-C-like DNA binding domain as a conserved feature of archaeal Rpa1-like proteins
MacNeill, S., 20 Jul 2021, In: Frontiers in Molecular Biosciences. 8, 8 p., 675229.Research output: Contribution to journal › Article › peer-review
Open AccessFile1 Citation (Scopus)6 Downloads (Pure) -
Non‐canonical binding of the Chaetomium thermophilum PolD4 N‐terminal PIP motif to PCNA involves Q‐pocket and compact 2‐fork plug interactions but no 310 helix
Yang, D., Alphey, M. S. & MacNeill, S., 22 Aug 2022, (E-pub ahead of print) In: FEBS Journal. Early View, 14 p., 16590.Research output: Contribution to journal › Article › peer-review
Open AccessFile1 Citation (Scopus)8 Downloads (Pure)
Datasets
-
Non‐canonical binding of the Chaetomium thermophilum PolD4 N‐terminal PIP motif to PCNA involves Q‐pocket and compact 2‐fork plug interactions but no 310 helix (dataset)
MacNeill, S. (Creator), Protein Data Bank (PDB), 29 Mar 2021
DOI: 10.2210/pdb7O1E/pdb, http://10.2210/pdb7O1E/pdb and one more link, http://doi.org/10.2210/pdb7O1F/pdb (show fewer)
Dataset
-
PCNA from Chaetomium thermophilum in complex with PolD3 peptide
Alphey, M. S. (Creator), Wolford, C. (Creator) & MacNeill, S. (Creator), RCSB-PDB, 2023
DOI: 10.2210/pdb8P9O/pdb, https://www.rcsb.org/structure/8P9O
Dataset
-
PCNA from Chaetomium thermophilum in complex with Fen1 peptide
Alphey, M. S. (Creator), Wolford, C. (Creator) & MacNeill, S. (Creator), RCSB-PDB, 2023
DOI: 10.2210/pdb8q7i/pdb, https://www.rcsb.org/structure/8Q7I
Dataset
Projects
- 5 Finished
-
Why do T5-like bacteriophages encode: Why do T5-like bacteriophages encode a split DNA ligase?
MacNeill, S. (PI)
1/10/20 → 30/09/22
Project: Standard
-
Stuart MacNeill ISSF3: ISSF3 round 5 - Stuart MacNeill
MacNeill, S. (PI)
1/04/20 → 31/07/20
Project: Standard
-
Dr Stuart MacNeill: Determining the three-dimensional architecture of a four-subunit eukaryotic DNA polymerase and complex
MacNeill, S. (PI)
1/08/17 → 30/11/18
Project: Standard
-
MCM Complex: Determining the macromolecular structure and cellular function of an alternative MCM complex
MacNeill, S. (PI)
31/10/14 → 30/10/17
Project: Standard
Activities
-
25th Biennial Evergreen International Phage Meeting
Stuart MacNeill (Participant)
6 Aug 2023 → 11 Aug 2023Activity: Participating in or organising an event types › Participation in or organising a conference
-
External examiner for PhD thesis
Stuart MacNeill (External examiner)
1 Aug 2023Activity: Examination types › External examination
-
VOM2023 (Viruses of Microbes 2023)
Stuart MacNeill (Participant)
3 Jul 2023 → 7 Jul 2023Activity: Participating in or organising an event types › Participation in or organising a conference
-
External examiner for PhD thesis
Stuart MacNeill (External examiner)
1 Jul 2023Activity: Examination types › External examination
-
Viruses of Microbes-UK meeting
Stuart MacNeill (Participant)
26 Jan 2023 → 27 Jan 2023Activity: Participating in or organising an event types › Participation in or organising a conference