Research output per year
Research output per year
- 44 results
Search results
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2024
Allosteric activation unveils protein-mass modulation of ATP phosphoribosyltransferase product release
Read, B., Mitchell, J. B. O. & da Silva, R. G., 6 Apr 2024, In: Communications Chemistry. 7, 13 p., 77.Research output: Contribution to journal › Article › peer-review
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Crystal structure, steady-state and pre-steady-state kinetics of Acinetobacter baumannii ATP phosphoribosyltransferase
Read, B., Cadzow, A., Alphey, M. S., Mitchell, J. B. O. & da Silva, R. G., 16 Jan 2024, In: Biochemistry. 63, 2, p. 230-240 11 p.Research output: Contribution to journal › Article › peer-review
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Human 2'-deoxynucleoside 5'-phosphate N-hydrolase 1: the catalytic roles of Tyr24 and Asp80
Carberry, A. E., Devi, S., Harrison, D. J. & da Silva, R. G., 2 Apr 2024, In: ChemBioChem. 25, 7, 10 p., e202400047.Research output: Contribution to journal › Article › peer-review
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Snapshots of the reaction coordinate of a thermophilic 2'-deoxyribonucleoside/ribonucleoside transferase
Tang, P., Harding, C. J., Dickson, A., da Silva, R. G., Harrison, D. J. & Melo Czekster, C., 1 Mar 2024, In: ACS Catalysis. 14, 5, p. 3090-3102 13 p.Research output: Contribution to journal › Article › peer-review
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2023
Catalytic cycle of the bifunctional enzyme phosphoribosyl-ATP pyrophosphohydrolase/phosphoribosyl-AMP cyclohydrolase
Fisher, G., Pecaver, E., Read, B., Leese, S., Laing, E., Dickson, A., Melo Czekster, C. & da Silva, R. G., 2 Jun 2023, In: ACS Catalysis. 13, 11, p. 7669-7679 11 p.Research output: Contribution to journal › Article › peer-review
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Human 2'-deoxynucleoside 5'-phosphate N-hydrolase 1: mechanism of 2'-deoxyuridine 5'-monophosphate hydrolysis
Devi, S., Carberry, A. E., Zickuhr, G. M., Dickson, A., Harrison, D. J. & da Silva, R. G., 5 Sept 2023, In: Biochemistry. 62, 17, p. 2658-2668 11 p.Research output: Contribution to journal › Article › peer-review
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2022
Allosteric inhibition of Acinetobacter baumannii ATP phosphoribosyltransferase by protein:dipeptide and protein:protein Interactions
Read, B. J., Fisher, G., Wissett, O. L. R., Machado, T. F. G., Nicholson, J., Mitchell, J. B. O. & da Silva, R. G., 14 Jan 2022, In: ACS Infectious Diseases. 8, 1, p. 197-209 13 p.Research output: Contribution to journal › Article › peer-review
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Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation
Fisher, G., Corbella, M., Alphey, M. S., Nicholson, J., Read, B., Kamerlin, S. C. L. & da Silva, R. G., 9 Dec 2022, In: Nature Communications. 13, 15 p., 7607.Research output: Contribution to journal › Article › peer-review
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Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m1A22-tRNA methyltransferase TrmK
Sweeney, P., Galliford, A., Kumar, A., Raju, D., Krishna, N. B., Sutherland, E., Leo, C. J., Fisher, G., Lalitha, R., Muthuraj, L., Sigamani, G., Oehler, V., Synowsky, S. A., Shirran, S. L., Gloster, T., Melo Czekster, C., Kumar, P. & da Silva, R. G., May 2022, In: Journal of Biological Chemistry. 298, 6, 15 p., 102040.Research output: Contribution to journal › Article › peer-review
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2021
Transition states for psychrophilic and mesophilic (R)-3-hydroxybutyrate dehydrogenase-catalyzed hydride transfer at sub-zero temperatures
Machado, T. F. G., Purg, M., Åqvist, J. & da Silva, R. G., 13 Jul 2021, In: Biochemistry. 60, 27, p. 2186–2194Research output: Contribution to journal › Article › peer-review
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2020
Dissecting the mechanism of (R)-3-hydroxybutyrate dehydrogenase by kinetic isotope effects, protein crystallography, and computational chemistry
G. Machado, T. F., Purg, M., McMahon, S., Read, B., Oehler, V., Åqvist, J., Gloster, T. & da Silva, R. G., 18 Dec 2020, In: ACS Catalysis. 10, 24, p. 15019–15032Research output: Contribution to journal › Article › peer-review
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2019
An exceptionally potent inhibitor of human CD73
Bowman, C. E., da Silva, R. G., Pham, A. & Young, S. W., 6 Aug 2019, In: Biochemistry. 58, 31, p. 3331-3334 4 p.Research output: Contribution to journal › Article › peer-review
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Mapping the structural path for allosteric inhibition of a short-form ATP phosphoribosyltransferase by histidine
Thomson, C. M., Alphey, M. S., Fisher, G. & da Silva, R. G., 16 Jul 2019, In: Biochemistry. 58, 28, p. 3078-3086Research output: Contribution to journal › Article › peer-review
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2018
Allosteric activation shifts the rate-limiting step in a short-form ATP phosphoribosyltransferase
Fisher, G., Thomson, C. M., Stroek, R., Czekster, C. M., Hirschi, J. S. & da Silva, R. G., 24 Jul 2018, In: Biochemistry. 57, 29, p. 4357-4367Research output: Contribution to journal › Article › peer-review
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Catalytic and anticatalytic snapshots of a short-form ATP phosphoribosyltransferase
Alphey, M. S., Fisher, G., Ge, Y., Gould, E. R., Guerreiro Machado, T. F., Liu, H., Florence, G. J., Naismith, J. H. & da Silva, R. G., 1 Jun 2018, In: ACS Catalysis. 8, 6, p. 5601-5610 10 p.Research output: Contribution to journal › Article › peer-review
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Discovery of trifluoromethyl glycol carbamates as potent and selective covalent monoacylglycerol lipase (MAGL) inhibitors for treatment of neuroinflammation
McAllister, L., Butler, C., Mente, S., O'Neil, S. V., Fonseca, K., Piro, J., Cianfrogna, J., Foley, T., Gilbert, A., Harris, A., Helal, C. J., Johnson, D. S., Montgomery, J. I., Nason, D. M., Noell, S., Pandit, J., Rogers, B. N., Samad, T. A., Shaffer, C. L. & da Silva, R. G. & 3 others, , 12 Apr 2018, In: Journal of Medicinal Chemistry. 61, 7, p. 3008–3026Research output: Contribution to journal › Article › peer-review
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Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction
Machado, T. F. G., Gloster, T. & da Silva, R. G., 11 Dec 2018, In: Biochemistry. 57, 49, p. 6757-6761Research output: Contribution to journal › Article › peer-review
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2017
Kinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferase
Stroek, R., Ge, Y., Talbot, P. D., Glok, M. K., Bernas, K. E., Thomson, C. M., Gould, E. R., Alphey, M. S., Liu, H., Florence, G. J., Naismith, J. H. & da Silva, R. G., 7 Feb 2017, In: Biochemistry. 56, 5, p. 793-803 11 p.Research output: Contribution to journal › Article › peer-review
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2014
A continuous and direct assay to monitor leucine-rich repeat kinase 2 activity
Silva, R. G., Geoghegan, K. F., Qiu, X. & Aulabaugh, A., 2014, In: Analytical Biochemistry. 450, 1, p. 63-69Research output: Contribution to journal › Article › peer-review
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2013
Selective mitochondrial staining with small fluorescent probes: importance, design, synthesis, challenges and trends for new markers
Neto, B. A., Correa, J. R. & Silva, R. G., 2013, In: RSC Advances. 3, p. 5291-5301Research output: Contribution to journal › Article › peer-review
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The Biginelli reaction with an imidazolium-tagged recyclable iron catalyst: kinetics, mechanism, and antitumoral activity
Ramos, L. M., Guido, B. C., Nobrega, C. C., Correa, J. R., Silva, R. G., de Oliveira, H. C., Gomes, A. F., Gozzo, F. C. & Neto, B. A., 2013, In: Chemistry - A European Journal. 19, p. 4156-4168Research output: Contribution to journal › Article › peer-review
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2012
Constrained bonding environment in the Michaelis complex of Trypanosoma cruzi uridine phosphorylase
Silva, R. G., Kipp, D. R. & Schramm, V. L., 2012, In: Biochemistry. 51, p. 6715-6717Research output: Contribution to journal › Article › peer-review
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Synthesis, properties and highly selective mitochondria staining with novel, stable and superior benzothiadiazole fluorescent probes
Neto, B. A., Carvalho, P. H., Santos, D. C., Gatto, C. C., Ramos, L. M., de Vasconcelos, N. M., Correa, J. R., Costa, M. B., de Oliveira, H. C. & Silva, R. G., 2012, In: RSC Advances. 2, p. 1524-1532Research output: Contribution to journal › Article › peer-review
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2011
Arsenate and phosphate as nucleophiles at the transition states of human purine nucleoside phosphorylase
Silva, R. G., Hirschi, J. S., Ghanem, M., Murkin, A. S. & Schramm, V. L., 2011, In: Biochemistry. 50, p. 2701-2709Research output: Contribution to journal › Article › peer-review
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Femtosecond dynamics coupled to chemical barrier crossing in a Born-Oppenheimer enzyme
Silva, R. G., Murkin, A. S. & Schramm, V. L., 2011, In: Proceedings of the National Academy of Sciences of the United States of America. 108, p. 18661-18665Research output: Contribution to journal › Article › peer-review
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Mass-dependent bond vibrational dynamics influence catalysis by HIV-1 protease
Kipp, D. R., Silva, R. G. & Schramm, V. L., 2011, In: Journal of the American Chemical Society. 133, p. 19358-19361Research output: Contribution to journal › Article › peer-review
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Transition-state analysis of Trypanosoma cruzi uridine phosphorylase-catalyzed arsenolysis of uridine
Silva, R. G., Vetticatt, M. J., Merino, E. F., Cassera, M. B. & Schramm, V. L., 2011, In: Journal of the American Chemical Society. 133, p. 9923-9931Research output: Contribution to journal › Article › peer-review
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Uridine phosphorylase from Trypanosoma cruzi: kinetic and chemical mechanisms
Silva, R. G. & Schramm, V. L., 2011, In: Biochemistry. 50, p. 9158-9166Research output: Contribution to journal › Article › peer-review
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2010
Purine nucleoside phosphorylase activity and expression are upregulated in sites affected by periodontal disease
Batista Jr, E. L., Deves, C., Ayub, L., da Silva, R. G., Filho, L., Basso, L. A. & Santos, D. S., 2010, In: Journal of Periodontal Research. 45, p. 664–671Research output: Contribution to journal › Article › peer-review
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2008
Mycobacterium tuberculosis beta-ketoacyl-ACP reductase: alpha-Secondary kinetic isotope effects and kinetic and equilibrium mechanisms of substrate binding
Silva, R. G., Rosado, L., Santos, D. S. & Basso, L. A., 2008, In: Archives of Biochemistry and Biophysics. 471, p. 1-10Research output: Contribution to journal › Article › peer-review
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2007
Kinetic and chemical mechanisms of shikimate dehydrogenase from Mycobacterium tuberculosis
Fonseca, I. O., Silva, R. G., Fernandes, C., de Souza, O. N., Basso, L. A. & Santos, D. S., 2007, In: Archives of Biochemistry and Biophysics. 457, p. 123-133Research output: Contribution to journal › Article › peer-review
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Purine Nucleoside Phosphorylase: A Potential Target for the Development of Drugs to Treat T-Cell- and Apicomplexan Parasite-Mediated Diseases
Silva, R. G., Nunes, J. E. S., Canduri, F., Borges, J. C., Gava, L. M., Moreno, F. B., Basso, L. A. & Santos, D. S., 2007, In: Current Drug Targets. 8, p. 413-422Research output: Contribution to journal › Article › peer-review
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2006
Beta-Ketoacyl-Acyl Carrier Protein (ACP) Reductase: Kinetic and Chemical Mechanisms
Silva, R. G., de Carvalho, L. P. S., Blanchard, J. S., Santos, D. S. & Basso, L. A., 2006, In: Biochemistry. 45, p. 13064-13073Research output: Contribution to journal › Article › peer-review
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Functional shikimate dehydrogenase from Mycobacterium tuberculosis H37Rv: Purification and characterization
Fonseca, I. O., Magalhaes, M. L. B., Oliveira, J. S., Silva, R. G., Mendes, M. A., Palma, M. S., Santos, D. S. & Basso, L. A., 2006, In: Protein Expression and Purification. 46, p. 429-437Research output: Contribution to journal › Article › peer-review
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2005
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine
Silva, R. G., Pereira, J. H., Canduri, F., de Azevedo, W., Basso, L. & Santos, D., 2005, In: Archives of Biochemistry and Biophysics. 442, p. 49-58Research output: Contribution to journal › Article › peer-review
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Structure of human PNP complexed with ligands
Canduri, F., Silva, R. G., dos Santos, D. M., Palma, M., Basso, L., Santos, D. & de Azevedo, W., 2005, In: Acta Crystallographica. Section D, Biological crystallography. 61, p. 856-862Research output: Contribution to journal › Article › peer-review
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2004
Purine Nucleoside Phosphorylase Activity in Rat Cerebrospinal Fluid
Silva, R. G., Santos, D., Basso, L., Oses, J., Wofchuk, S., Portela, L. & Souza, D., 2004, In: Neurochemical Research. 29, p. 1831-1835Research output: Contribution to journal › Article › peer-review
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Structures of human purine nucleoside phosphorylase complexed with inosine and ddI
Canduri, F., dos Santos, D., Silva, R. G., Mendes, M., Basso, L., MS, P., de Azevedo, W. & Santos, D., 2004, In: Biochemical and Biophysical Research Communications. 313, p. 907-914Research output: Contribution to journal › Article › peer-review
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2003
Cloning, overexpression, and purification of functional human purine nucleoside phosphorylase
Silva, R. G., de Carvalho, L., Oliveira, J., Pinto, C., Mendes, M., Palma, M., Basso, L. & Santos, D., 2003, In: Protein Expression and Purification. 27, p. 158-164Research output: Contribution to journal › Article › peer-review
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Crystal structure of human PNP complexed with guanine
de Azevedo, W., Canduri, F., dos Santos, D., Pereira, J., Dias, M., Silva, R. G., Mendes, M., Basso, L., Palma, M. & Santos, D., 2003, In: Biochemical and Biophysical Research Communications. 312, p. 767-772Research output: Contribution to journal › Article › peer-review
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Crystal structure of human purine nucleoside phosphorylase at 2.3 A resolution
de Azevedo, W., Canduri, F., dos Santos, D., Silva, R. G., Oliveira, J., de Carvalho, L., Basso, L., Mendes, M., Palma, M. & Santos, D., 2003, In: Biochemical and Biophysical Research Communications. 308, p. 545-552Research output: Contribution to journal › Article › peer-review
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Crystal structure of human purine nucleoside phosphorylase complexed with acyclovir
dos Santos, D., Canduri, F., Pereira, J., Dias, M., Silva, R. G., Mendes, M., Palma, M., Basso, L., de Azevedo, W. & Santos, D., 2003, In: Biochemical and Biophysical Research Communications. 308, p. 553-559Research output: Contribution to journal › Article › peer-review
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Docking and small angle X-ray scattering studies of purine nucleoside phosphorylase
de Azevedo, W., dos Santos, G., dos Santos, D., Olivieri, J., Canduri, F., Silva, R. G., Basso, L., Renard, G., Fonseca, I., Mendes, M., Palma, M. & Santos, D., 2003, In: Biochemical and Biophysical Research Communications. 309, p. 923-928Research output: Contribution to journal › Article › peer-review
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Structural basis for inhibition of human PNP by immucillin-H
de Azevedo, W., Canduri, F., dos Santos, D., Pereira, J., Dias, M., Silva, R. G., Mendes, M., Basso, L., Palma, M. & Santos, D., 2003, In: Biochemical and Biophysical Research Communications. 309, p. 917–922Research output: Contribution to journal › Article › peer-review