Modular nanoparticle platform for solution-phase optical sensing of protein–protein interactions (dataset)

Scanning transmission electron microscopy (STEM) data including energy dispersive X-ray spectroscopy (EDX) and electron energy loss spectroscopy (EELS) data.

file type(s): uncompressed 16-bit TIFFs, EDX line profiles as CSVs, EEL spectra as MSAs (type of text file with a header).
open with: TIFFs - any image editing software; CSVs/MSAs - any software capable of handling text files (e.g. text editors, LibreOffice Calc, Python, Matlab, Origin, etc.).

metadata: TIFFs - stored in headers, can be read using e.g. ImageJ/Fiji (https://imagej.net/formats/metadata); MSAs - stored in text headers

acquisition details: "STEM was performed on a Titan Themis (FEI) operated at 200 kV and equipped with a DCOR probe corrector (CEOS), a SuperX energy dispersive X-ray spectrometer (EDX), and a Gatan Enfinium electron energy loss spectrometer (EELS). High angle annular dark field (HAADF) images were acquired with a probe convergence angle of 21.2 mrad and inner/outer collection angles of 74 and 200 mrad, respectively. EELS spectra were acquired with a collection angle of 8.1 mrad; the background was subtracted using a power-law background, and the spectra were corrected for plural scattering by Fourier ratio deconvolution."